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用于筛查腹泻粪便以培养艰难梭菌的培养基比较

Comparison of media for screening of diarrheic stools for the recovery of Clostridium difficile.

作者信息

Iwen P C, Booth S J, Woods G L

机构信息

Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha 68105.

出版信息

J Clin Microbiol. 1989 Sep;27(9):2105-6. doi: 10.1128/jcm.27.9.2105-2106.1989.

DOI:10.1128/jcm.27.9.2105-2106.1989
PMID:2778074
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC267748/
Abstract

Recoveries of Clostridium difficile from stool specimens by using three media, cycloserine-mannitol agar (M-CMA), cycloserine-mannitol-blood agar (M-CMBA), and cycloserine-cefoxitin agar (M-CCA), were compared. Of 321 clinical specimens, 37 yielded C. difficile. Thirty-four were positive on M-CCA, 21 were positive on M-CMA, and 20 were positive on M-CMBA. M-CCA recovered significantly more C. difficile than did M-CMBA or M-CMA.

摘要

比较了使用三种培养基（环丝氨酸甘露醇琼脂（M-CMA）、环丝氨酸甘露醇血琼脂（M-CMBA）和环丝氨酸头孢西丁琼脂（M-CCA））从粪便标本中分离艰难梭菌的情况。在321份临床标本中，37份培养出艰难梭菌。34份在M-CCA上呈阳性，21份在M-CMA上呈阳性，20份在M-CMBA上呈阳性。M-CCA分离出的艰难梭菌显著多于M-CMBA或M-CMA。

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本文引用的文献

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Relative frequency of Clostridium difficile in patients with diarrheal disease.腹泻病患者中艰难梭菌的相对频率。
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Anaerobic infections and Clostridium difficile colitis emerging during antibacterial therapy.抗菌治疗期间出现的厌氧菌感染和艰难梭菌结肠炎。
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Comparison of Minitek Anaerobe II, API An-Ident, and RapID ANA systems for identification of Clostridium difficile.用于艰难梭菌鉴定的Minitek厌氧II型系统、API An-Ident系统和RapID ANA系统的比较
Am J Clin Pathol. 1986 Jun;85(6):716-8. doi: 10.1093/ajcp/85.6.716.
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J Clin Microbiol. 1988 Mar;26(3):540-3. doi: 10.1128/jcm.26.3.540-543.1988.
10
Immunoblots and plasmid fingerprints compared with serotyping and polyacrylamide gel electrophoresis for typing Clostridium difficile.免疫印迹法和质粒指纹图谱与血清分型及聚丙烯酰胺凝胶电泳用于艰难梭菌分型的比较。
J Clin Microbiol. 1988 Jan;26(1):41-6. doi: 10.1128/jcm.26.1.41-46.1988.