Sánchez-Sampedro Maria Angeles, Fernández-Tárrago Jorge, Corchete Purificación
Department of Plant Physiology, Faculty of Pharmacy, University of Salamanca, Campus Miguel de Unamuno, 37007, Salamanca, Spain.
Biotechnol Lett. 2009 Oct;31(10):1633-7. doi: 10.1007/s10529-009-0043-0. Epub 2009 Jun 6.
Production of silymarin and the effect of the elicitor, methyl jasmonate (MeJA), was monitored in cell cultures of Silybum marianum over 4 years. Silymarin concentrations gradually declined after prolonged subculture, making the success of elicitor strategy limited in long-term cultures. The continuous presence of MeJA in cultures for an extended period was necessary for induction of silymarin accumulation. A repeated elicitor strategy was not a good option for improving silymarin productivity in batch cultures. Removal of medium from elicited cultures and addition of fresh medium avoided the toxic effects of elicitor accumulation, allowing the system to respond to a repeated MeJA treatment without loss of productivity.
在4年的时间里，对水飞蓟宾的产生以及茉莉酸甲酯（MeJA）这种诱导子的作用在水飞蓟的细胞培养物中进行了监测。经过长时间的继代培养后，水飞蓟宾浓度逐渐下降，这使得诱导子策略在长期培养中的成功受到限制。在培养物中长时间持续存在MeJA对于诱导水飞蓟宾积累是必要的。对于提高分批培养中水飞蓟宾的生产力而言，重复诱导子策略并非一个好的选择。从诱导培养物中去除培养基并添加新鲜培养基可避免诱导子积累的毒性作用，使该系统能够对重复的MeJA处理做出反应而不损失生产力。
