Department of Biology, Faculty of Science, Tarbiat Moallem University, Tehran, Iran.
Nat Prod Res. 2012;26(18):1662-7. doi: 10.1080/14786419.2011.593518. Epub 2011 Oct 11.
In this article our aim was to evaluate mass cultivation of S. marianum hairy roots in a bioreactor to produce silymarin. The effects of methyl jasmonate (MJ) elicitation on the accumulation of silymarin and the extent of the MJ-induced oxidative damage were investigated in bioreactor hairy root cultures of S. marianum. The growth rate of the bioreactor hairy root cultures was higher than that of those in a shake flask after 3 weeks. Silymarin accumulation was increased from 0.13 mg g⁻¹ DW in non-treated hairy roots to 0.22 mg g⁻¹ DW in hairy roots 72 h after 100 µM MJ treatment. Guaiacol peroxidase and ascorbate peroxidase were activated by MJ 72 h after treatment, being 3.2- and 1.3-fold higher, respectively, than that of the control. An increase in enzymatic activity suggests increased scavenging of reactive oxygen species, indicating the tolerance to MJ stress. These results suggest that MJ elicitation is beneficial for silymarin production using bioreactor hairy root cultures.
本文旨在评估在生物反应器中大规模培养水飞蓟发根以生产水飞蓟素。研究了茉莉酸甲酯（MJ）对水飞蓟发根中水飞蓟素积累和 MJ 诱导的氧化损伤程度的影响。与摇瓶培养相比，生物反应器培养 3 周后，生物反应器发根的生长速度更快。在 100μM MJ 处理 72 小时后，未处理发根中的水飞蓟素积累从 0.13mg/g DW 增加到 0.22mg/g DW。处理 72 小时后，愈创木酚过氧化物酶和抗坏血酸过氧化物酶被 MJ 激活，分别比对照高出 3.2 倍和 1.3 倍。酶活性的增加表明活性氧的清除增加，表明对 MJ 胁迫的耐受性。这些结果表明，茉莉酸甲酯诱导有利于生物反应器发根培养生产水飞蓟素。
