Department of Emergency Medicine, Selçuk University, Konya, Turkey.
Hum Exp Toxicol. 2013 Jan;32(1):45-52. doi: 10.1177/0960327112455070. Epub 2012 Oct 11.
The aim of this study was to examine the effects of ubiquinone (CoQ10) on heart tissue and erythrocytes in acute organophosphate poisoning (AOP). A total of 20 rabbits were divided into three groups: sham (n = 8), pralidoxime (PAM) + atropine (n = 6), and CoQ10 + PAM + atropine (n = 6). Blood samples were taken from each test subject to measure the values of acetylcholinesterase (AChE), nitric oxide (NO), and malondialdehyde (MDA) in the plasma and erythrocyte before administration of 50 mg/kg dichlorvos by orogastric tube. Blood samples were then taken at 1, 12, and 24 h post-dichlorvos to determine plasma and erythrocyte levels of AChE, NO, and MDA. Sham group received no treatment. PAM + atropine group received 0.05 mg/kg atropine with repeated doses and PAM: first a 30-mg/kg intravenous (IV) bolus, then a 15-mg/kg IV bolus every 4 h. CoQ10 + PAM + atropine group received same dose PAM and atropine and a 50-mg bolus of IV CoQ10. Thoracotomy was performed in all the animals 24 h after poisoning and then heart tissue samples were obtained. At 12 and 24 h, erythrocyte AChE levels in the CoQ10 animals were considerably higher than those in PAM + atropine animals (p = 0.023 and 0.017, respectively). At 12 and 24 h, erythrocyte MDA and NO levels in CoQ10 animals were significantly lower than those in PAM + atropine animals (p < 0.05). Heart tissue AChE levels in CoQ10 animals were considerably higher than those of the sham and PAM + atropine animals (p = 0.001). Heart tissue MDA and NO levels of CoQ10 animals were significantly lower than those of the sham and PAM + atropine animals (p < 0.01). Treatment of AOP with CoQ10 + PAM + atropine in this animal model had a beneficial effect on both erythrocyte and heart tissue lipid peroxidation and AChE activity.
本研究旨在探讨泛醌（CoQ10）对急性有机磷中毒（AOP）心脏组织和红细胞的影响。将 20 只兔子分为三组：假手术组（n = 8）、氯解磷定（PAM）+阿托品（n = 6）和 CoQ10+PAM+阿托品（n = 6）。在通过口胃管给予 50mg/kg 敌敌畏之前，从每个受试动物抽取血样，以测量血浆和红细胞中的乙酰胆碱酯酶（AChE）、一氧化氮（NO）和丙二醛（MDA）的值。然后在敌敌畏后 1、12 和 24 小时抽取血样，以确定血浆和红细胞中的 AChE、NO 和 MDA 水平。假手术组未进行任何治疗。PAM+阿托品组给予 0.05mg/kg 阿托品，并重复给予剂量，以及 PAM：首先给予 30mg/kg 静脉（IV）推注，然后每 4 小时给予 15mg/kg IV 推注。CoQ10+PAM+阿托品组给予相同剂量的 PAM 和阿托品，以及 50mg/kg IV CoQ10 推注。所有动物在中毒后 24 小时行开胸术，然后获取心脏组织样本。在 12 和 24 小时时，CoQ10 动物的红细胞 AChE 水平明显高于 PAM+阿托品动物（分别为 p = 0.023 和 0.017）。在 12 和 24 小时时，CoQ10 动物的红细胞 MDA 和 NO 水平明显低于 PAM+阿托品动物（p < 0.05）。CoQ10 动物的心脏组织 AChE 水平明显高于假手术和 PAM+阿托品动物（p = 0.001）。CoQ10 动物的心脏组织 MDA 和 NO 水平明显低于假手术和 PAM+阿托品动物（p < 0.01）。在该动物模型中，用 CoQ10+PAM+阿托品治疗 AOP 对红细胞和心脏组织脂质过氧化和 AChE 活性均有有益作用。
