Stratmann U, Schaarschmidt K, Lehmann R, Heinze A, Willital G H, Störmann J, Wessling G
Anatomical Institute, University of Münster, FRG.
Acta Anat (Basel). 1991;141(1):85-9. doi: 10.1159/000147104.
36 rat esophagi were irradiated by argon laser via an applicator with circumferential light distribution. They were perfused with glutaraldehyde and studied by light and transmission electron microscopy immediately, 2 days and 14 days after irradiation. Immediately after irradiation the laser center showed destruction of the keratinized stratified squamous epithelium. The collagenous fibers of the connective tissue were altered; fibrocytes and fibroblasts were severely damaged, and the microvascular lumina were occluded. The smooth muscle tissue and skeletal muscle tissue showed myofilament defects and initial karyonecrosis. There was decreasing damage of both fiber types up to 4 mm from the laser center. After 2 days the morphology of the laser center was not different from that seen immediately after irradiation. At a distance of 2 mm a partly differentiated new epithelium emerged below the necrotic epithelium. An inflammatory reaction was found in the connective tissue. After 14 days the esophageal wall was replaced and the lumen was occluded by young granulation tissue in the former laser center. Peripherally the esophageal wall appeared almost normal. As the rat esophagus serves as a model for esophagotracheal fistulae in newborn children, our findings indicate that the argon laser should be capable of occluding these fistulae likewise.
36只大鼠食管通过具有周向光分布的施照器接受氩激光照射。照射后立即、2天和14天分别用戊二醛灌注，并进行光镜和透射电镜研究。照射后立即，激光中心显示角化复层鳞状上皮破坏。结缔组织的胶原纤维发生改变；纤维细胞和成纤维细胞严重受损，微血管腔闭塞。平滑肌组织和骨骼肌组织出现肌丝缺陷和初期核坏死。距激光中心4毫米范围内两种纤维类型的损伤均逐渐减轻。2天后，激光中心的形态与照射后立即观察到的无异。在距坏死上皮下方2毫米处出现部分分化的新上皮。在结缔组织中发现炎症反应。14天后，食管壁被新生肉芽组织取代，原激光中心管腔闭塞。周边食管壁几乎正常。由于大鼠食管可作为新生儿食管气管瘘的模型，我们的研究结果表明氩激光同样能够闭塞这些瘘管。
