Russek-Blum Niva, Nabel-Rosen Helit, Levkowitz Gil
Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot, Israel.
Dev Dyn. 2009 Jul;238(7):1827-35. doi: 10.1002/dvdy.21987.
The diencephalon acts as an interactive site between the sensory, central, and endocrine systems and is one of the most elaborate structures in the vertebrate brain. To better understand the embryonic development and morphogenesis of the diencephalon, we developed an improved photoactivation (uncaging)-based lineage tracing strategy. To determine the exact position of a given diencephalic progenitor domain, we used a transgenic line driving green fluorescent protein (GFP) in cells expressing the proneural protein, Neurogenin1 (Neurog1), which was used as a visible neural plate landmark. This approach facilitated precise labeling of defined groups of cells in the prospective diencephalon of the zebrafish neural plate. In this manner, we labeled multiple overlapping areas of the diencephalon, thereby ensuring both accuracy and reproducibility of our lineage tracing regardless of the dynamic changes of the developing neural plate. We present a fate map of the zebrafish diencephalon at a higher spatial resolution than previously described.
间脑是感觉、中枢和内分泌系统之间的一个交互部位，是脊椎动物大脑中最复杂的结构之一。为了更好地理解间脑的胚胎发育和形态发生，我们开发了一种改进的基于光激活（解笼）的谱系追踪策略。为了确定给定间脑祖细胞区域的精确位置，我们使用了一个转基因品系，该品系在表达神经前体蛋白Neurogenin1（Neurog1）的细胞中驱动绿色荧光蛋白（GFP）表达，Neurog1被用作可见的神经板标志物。这种方法有助于在斑马鱼神经板的预期间脑中对特定细胞群进行精确标记。通过这种方式，我们标记了间脑的多个重叠区域，从而确保了我们谱系追踪的准确性和可重复性，而不受发育中神经板动态变化的影响。我们展示了一个比之前描述的更高空间分辨率的斑马鱼间脑命运图谱。
