Park Myung Ryeol, Kim Young Cheol, Lee Sunwoo, Kim In Seon
Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Environmentally Friendly Agriculture Research Centre, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
Pest Manag Sci. 2009 Oct;65(10):1114-7. doi: 10.1002/ps.1800.
Klebsiella oxytoca C1036 (C1036) causes induced systemic resistance (ISR) activity against the soft-rot pathogen Pectobacterium carotovorum subsp. carotovorum SCC1 (SCC1). However, microbial metabolites from C1036 involved in ISR activity remain unknown. The present study was performed to identify an ISR-related metabolite produced by C1036.
The supernatants of C1036 cultures grown on Luria-Bertani medium were subjected to solvent extraction, repeated column chromatography and preparative liquid chromatography for isolation of an ISR-related metabolite. High-resolution mass spectrometer analysis of the isolated metabolite indicated a C9H15O3N compound with a mass of 185.11. Low-resolution mass spectrometer analysis of the metabolite showed a molecular ion peak at 185 and its fragment ions at 84 and 56. Nuclear magnetic resonance spectrometer analyses characterised all protons and carbons of the isolated metabolite. Based on the data, the isolated metabolite was determined to be butyl 2-pyrrolidone-5-carboxylate (BPC). BPC at 12 mM significantly suppressed the disease symptoms in ISR bioassays against SCC1.
This is the first report identifying BPC as an ISR-related metabolite produced by C1036. C1036 may play a role in promoting plant growth because it produces ISR-related metabolites against the plant pathogen SCC1.
产酸克雷伯菌C1036（C1036）对软腐病原菌胡萝卜软腐果胶杆菌胡萝卜亚种SCC1（SCC1）具有诱导系统抗性（ISR）活性。然而，C1036中参与ISR活性的微生物代谢产物尚不清楚。本研究旨在鉴定C1036产生的一种与ISR相关的代谢产物。
将在Luria-Bertani培养基上生长的C1036培养物上清液进行溶剂萃取、反复柱色谱和制备液相色谱，以分离与ISR相关的代谢产物。对分离出的代谢产物进行高分辨率质谱分析，结果表明该化合物为C9H15O3N，分子量为185.11。对该代谢产物进行低分辨率质谱分析，结果显示其分子离子峰为185，碎片离子峰为84和56。核磁共振光谱分析确定了分离出的代谢产物的所有质子和碳。基于这些数据，确定分离出的代谢产物为2-吡咯烷酮-5-羧酸丁酯（BPC）。在针对SCC1的ISR生物测定中，12 mM的BPC显著抑制了病害症状。
这是首次报道鉴定BPC为C1036产生的与ISR相关的代谢产物。C1036可能在促进植物生长方面发挥作用，因为它能产生针对植物病原菌SCC1的与ISR相关的代谢产物。
