Chen Xiaoqi, Mou Yanhua, Ling Junhong, Wang Nan, Wang Xiao, Hu Jiangchun
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110016, China.
World J Microbiol Biotechnol. 2015 Jan;31(1):247-53. doi: 10.1007/s11274-014-1759-0. Epub 2014 Oct 25.
Extracellular alkalinization and H2O2 production are important early events during induced systemic resistance (ISR) establishment in plants. In a screen for metabolites as potential ISR activators from 98 fungal isolates associated with marine sponge Hymeniacidon perleve, the crude metabolites of fungus Eupenicillium brefeldianum HMP-F96 induced significant extracellular alkalinization coupled with H2O2 production in tobacco cell suspensions. A combined bioactivity and (1)H NMR-guided fractionation approach was used to disclose the chemical determinants responsible for the activities. Eight cyclic dipeptides were purified from the fermentation broth of the strain and were structurally characterized by NMR and MS experiments. This study represents the first report of the occurrence of cyclic dipeptides in E. brefeldianum and of their activities of inducing extracellular alkalinization and H2O2 production in tobacco cell suspensions.
细胞外碱化和过氧化氢生成是植物诱导系统抗性（ISR）建立过程中的重要早期事件。在一项从与海鞘状海绵 Hymeniacidon perleve 相关的 98 种真菌分离物中筛选潜在 ISR 激活剂代谢物的研究中，真菌短柄帚霉 HMP-F96 的粗代谢产物在烟草细胞悬浮液中诱导了显著的细胞外碱化并伴有过氧化氢生成。采用生物活性与¹H NMR 导向分级分离相结合的方法来揭示负责这些活性的化学决定因素。从该菌株的发酵液中纯化出了 8 种环二肽，并通过 NMR 和 MS 实验对其进行了结构表征。本研究首次报道了环二肽在短柄帚霉中的存在及其在烟草细胞悬浮液中诱导细胞外碱化和过氧化氢生成的活性。
