Shirosaki Yuki, Botelho Cláudia M, Lopes Maria A, Santos José D
Faculdade de Engenharia da Universidade do Porto, Seccáo de Materiais, DEMM, Rua Dr Roberto Frias, 4200-465, Porto, Portugal.
J Nanosci Nanotechnol. 2009 Jun;9(6):3714-9. doi: 10.1166/jnn.2009.ns56.
The use of bone grafts is required to restore skeletal integrity and enhance bone healing of large defects in several areas of regenerative medicine, such as: orthopedic and maxillofacial procedures. Some of these bone grafts can be resorbed in a time controlled way, in order to allow the correct process of natural re-construction of the involved bone tissue to occur. The Bonelike graft is a bone substitute that mimics the inorganic composition of bone; this biomaterial was developed and characterized over the last decade. In a granular form, Bonelike has proved its highly bioactive behavior in medical applications, such as; maxillofacial and orthopedics surgery. The clinical applications in maxillary bone defects indicated a good bone bonding between new formed bone and the Bonelike granules. The purpose of this study was to develop a new injectable system for the application of Bonelike using a resorbable vehicle which may be used in minimal invasive surgery. A new hydrogel derived from chitosan and y-glycidoxypropyltrimethoxysilane (GPTMS) was synthesized and characterized. The mixture derived from chitosan and GPTMS existed in sol state at room temperature and formed a hydrogel at 37 degrees C. The degradability of the hydrogel could be controlled by the concentration of chitosan and GPTMS, and the presence the presence of Bonelike did not affect its degradability. The pH changes caused by the degradation of this hydrogel were small, so it is not expected to cause any deleterious effect in vivo conditions.
在再生医学的多个领域，如骨科和颌面手术中，需要使用骨移植来恢复骨骼完整性并促进大骨缺损的愈合。其中一些骨移植材料可以以时间可控的方式被吸收，以便使相关骨组织的自然重建过程正确发生。骨样移植物是一种模仿骨无机成分的骨替代物；这种生物材料在过去十年中得到了开发和表征。呈颗粒状的骨样移植物在医学应用（如颌面和骨科手术）中已证明具有高度的生物活性。在上颌骨缺损的临床应用中，新形成的骨与骨样颗粒之间显示出良好的骨结合。本研究的目的是开发一种新的可注射系统，用于使用可吸收载体应用骨样移植物，该系统可用于微创手术。合成并表征了一种由壳聚糖和γ-缩水甘油醚氧丙基三甲氧基硅烷（GPTMS）衍生的新型水凝胶。壳聚糖和GPTMS的混合物在室温下呈溶胶状态，在37℃时形成水凝胶。水凝胶的降解性可通过壳聚糖和GPTMS的浓度来控制，并且骨样移植物的存在不影响其降解性。这种水凝胶降解引起的pH变化很小，因此预计在体内条件下不会造成任何有害影响。
