Department of Materials Science, Faculty of Science, Chulalongkorn University, Phayathai, Pathumwan, Bangkok 10330, Thailand.
Department of Biochemistry, Faculty of Pharmacy, Mahidol University, 447 Sri-ayuthaya Road, Rajathevee, Bangkok 10400, Thailand.
Int J Biol Macromol. 2019 Feb 15;123:167-173. doi: 10.1016/j.ijbiomac.2018.11.041. Epub 2018 Nov 10.
This research aimed to design an injectable chitosan-based hydrogel for bone tissue engineering. To this end, chitosan should not only be soluble in water but also be thixotropic. Therefore, it can be used as an injectable liquid that can form a hydrogel under physiological conditions. Accordingly, chitosan was modified with carboxymethyl chloride and the resultant carboxymethyl chitosan (CMCTS) was conjugated to α-cyclodextrin (α-CD) to yield CMCTSCD. The α-CD conjugation improved the water solubility and the hydrophobic cavities to function as crosslinking points by forming an inclusion complex with poly(ethylene glycol) (PEG). The optimum mole ratio of α-CD (as CMCTSCD) to PEG was found to be 2 to 4 with a threading number of ~2.5. The sol-to-gel transition was formed within 450 ± 10 min. The interconnecting porous structure of the gel was observed by scanning electron microscope. Therefore, CMCTSCD/PEG is a potential novel design that can be used as an injectable hydrogel for bone tissue engineering.
本研究旨在设计一种可注射的壳聚糖基水凝胶用于骨组织工程。为此，壳聚糖不仅应溶于水，而且应为触变的。因此，它可用作可注射的液体，可在生理条件下形成水凝胶。相应地，壳聚糖用氯乙酸进行了改性，所得羧甲基壳聚糖（CMCTS）与α-环糊精（α-CD）偶联，得到 CMCTSCD。α-CD 的偶联提高了水溶性，并通过与聚乙二醇（PEG）形成包合物作为交联点，形成疏水性空腔。发现α-CD（作为 CMCTSCD）与 PEG 的最佳摩尔比为 2 至 4，穿线数约为 2.5。在 450±10min 内形成溶胶-凝胶转变。通过扫描电子显微镜观察到凝胶的互连多孔结构。因此，CMCTSCD/PEG 是一种潜在的新型设计，可用作用于骨组织工程的可注射水凝胶。
