Chen Min, Wu Jian, Zhang Xing-Xing, Wang Qiong, Yan Shi-Hai, Wang Hai-Dan, Liu Sheng-Lin, Zou Xi
General Internal Medicine Department, Jiangsu Hospital of Traditional Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, P.R. China.
Central Laboratory, Jiangsu Hospital of Traditional Chinese Medicine, The Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210029, P.R. China.
Oncol Lett. 2017 Jan;13(1):370-376. doi: 10.3892/ol.2016.5404. Epub 2016 Nov 21.
Sesquiterpenoids are a major type of compound found in ). The present study aimed to investigate whether sesquiterpenoids from demonstrated cytotoxicity against the MCF-7, HCT-8, A-549, SGC-7901 and BEL-7402 cell lines, and the mechanism of solajiangxin H and lyratol D, which exhibited high cytotoxicity against SGC-7901 cells (half maximal inhibitory concentration, IC=4.8 and 5.9 µg/ml), was associated with mitochondria-mediated apoptosis. The results of the Cell Counting Kit-8 assay indicated that 15 sesquiterpenoids had cytotoxicity against the aforementioned cultured cells. The results of DAPI staining and western blot analysis, used to study the anticancer mechanisms of solajiangxin H and lyratol D in SGC-7901 cells, suggested that solajiangxin H and lyratol D induced the apoptosis of SGC-7901 cells significantly (P<0.01), downregulated the expression of the antiapoptotic proteins B-cell lymphoma (Bcl)-2 and survivin, and upregulated the expression of the proapoptotic proteins Bcl-2-like protein 4, second mitochondria-derived activator of caspase, cleaved (c)-caspase-3 and c-caspase-9. The present study therefore demonstrated that 15 sesquiterpenoids from exhibited anticancer activity in MCF-7, HCT-8, A-549, SGC-7901 and BEL-7402 cells, and that the anticancer mechanisms of solajiangxin H and lyratol D may be associated with mitochondria-mediated apoptosis. Additionally, the present study provides evidence in support of the hypothesis that may be a promising candidate for the development of novel cancer therapies.
倍半萜类化合物是在（此处信息缺失）中发现的主要化合物类型。本研究旨在调查来自（此处信息缺失）的倍半萜类化合物是否对MCF - 7、HCT - 8、A - 549、SGC - 7901和BEL - 7402细胞系具有细胞毒性，以及对SGC - 7901细胞表现出高细胞毒性（半数最大抑制浓度，IC = 4.8和5.9 μg/ml）的索拉姜新H和赖百当醇D的作用机制是否与线粒体介导的凋亡相关。细胞计数试剂盒 - 8检测结果表明，15种倍半萜类化合物对上述培养细胞具有细胞毒性。用于研究索拉姜新H和赖百当醇D在SGC - 7901细胞中的抗癌机制的DAPI染色和蛋白质印迹分析结果表明，索拉姜新H和赖百当醇D显著诱导SGC - 7901细胞凋亡（P < 0.01），下调抗凋亡蛋白B细胞淋巴瘤（Bcl）- 2和存活素的表达，并上调促凋亡蛋白Bcl - 2样蛋白4、线粒体衍生的半胱天冬酶激活剂、裂解的（c）-半胱天冬酶 - 3和c -半胱天冬酶 - 9的表达。因此，本研究表明来自（此处信息缺失）的15种倍半萜类化合物在MCF - 7、HCT - 8、A - 549、SGC - 7901和BEL - 7402细胞中具有抗癌活性，并且索拉姜新H和赖百当醇D的抗癌机制可能与线粒体介导的凋亡相关。此外，本研究为（此处信息缺失）可能是开发新型癌症治疗方法的有前途的候选者这一假设提供了证据支持。
