Cunneen Monica M, De Castro Cristina, Kenyon Johanna, Parrilli Michelangelo, Reeves Peter R, Molinaro Antonio, Holst Otto, Skurnik Mikael
Division of Microbiology, School of Molecular and Microbial Biosciences, University of Sydney, Australia.
Carbohydr Res. 2009 Aug 17;344(12):1533-40. doi: 10.1016/j.carres.2009.04.030. Epub 2009 May 3.
In the Yersinia pseudotuberculosis serotyping scheme, 21 serotypes are present originating from about 30 different O-factors distributed within the species. With regard to the chemical structures of lipopolysaccharides (LPSs) and the genetic basis of their biosynthesis, a number, but not all, of Y. pseudotuberculosis strains representing different serotypes have been investigated. In order to present an overall picture of the relationship between genetics and structures, we have been working on the genetics and structures of various Y. pseudotuberculosis O-specific polysaccharides (OPSs). Here, we present a structural and genetic analysis of the Y. pseudotuberculosis serotype O:11 OPS. Our results showed that this OPS structure has the same backbone as that of Y. pseudotuberculosis O:1b, but with a 6d-L-Altf side-branch instead of Parf. The 3' end of the gene cluster is the same as that for O:1b and has the genes for synthesis of the backbone and for processing the completed repeat unit. The 5' end has genes for synthesis of 6d-L-Altf and its transfer to the repeating unit backbone. The pathway for the synthesis of the 6d-L-Altf appears to be different from that for 6d-L-Altp in Y. enterocolitica O:3. The chemical structure of the O:11 repeating unit is [structure: see text].
在假结核耶尔森菌血清分型方案中，共有21种血清型，源自该菌种内分布的约30种不同O因子。关于脂多糖（LPS）的化学结构及其生物合成的遗传基础，已对一些（但并非全部）代表不同血清型的假结核耶尔森菌菌株进行了研究。为了全面呈现遗传学与结构之间的关系，我们一直在研究各种假结核耶尔森菌O特异性多糖（OPS）的遗传学和结构。在此，我们展示了对假结核耶尔森菌血清型O:11 OPS的结构和遗传分析。我们的结果表明，这种OPS结构与假结核耶尔森菌O:1b具有相同的主链，但带有一个6d-L-Altf侧链而非Parf。基因簇的3'端与O:1b相同，具有合成主链和加工完整重复单元的基因。5'端具有合成6d-L-Altf及其转移至重复单元主链的基因。6d-L-Altf的合成途径似乎与小肠结肠炎耶尔森菌O:3中的6d-L-Altp不同。O:11重复单元的化学结构为[结构：见正文]。
