Division of Microbiology, School of Molecular Bioscience, University of Sydney, Sydney 2006, Australia.
Glycobiology. 2011 Sep;21(9):1131-9. doi: 10.1093/glycob/cwr006. Epub 2011 Feb 14.
The O-specific polysaccharide (OPS) is a variable constituent of the lipopolysaccharide of Gram-negative bacteria. The polymorphic nature of OPSs within a species is usually first defined serologically, and the current serotyping scheme for Yersinia pseudotuberculosis consists of 21 O serotypes of which 15 have been characterized genetically and structurally. Here, we present the structure and DNA sequence of Y. pseudotuberculosis O:10 OPS. The O unit consists of one residue each of d-galactopyranose, N-acetyl-d-galactosamine (2-amino-2-deoxy-d-galactopyranose) and d-glucopyranose in the backbone, with two colitose (3,6-dideoxy-l-xylo-hexopyranose) side-branch residues. This structure is very similar to that shared by Escherichia coli O111 and Salmonella enterica O35. The gene cluster sequences of these serotypes, however, have only low levels of similarity to that of Y. pseudotuberculosis O:10, although there is significant conservation of gene order. Within Y. pseudotuberculosis, the O10 structure is most closely related to the O:6 and O:7 structures.
O 特异性多糖 (OPS) 是革兰氏阴性菌脂多糖的可变成分。同一物种内 OPS 的多态性通常首先通过血清学定义，而当前的假结核耶尔森氏菌血清分型方案由 21 个 O 血清型组成，其中 15 个已在遗传和结构上进行了表征。在这里，我们展示了假结核耶尔森氏菌 O:10 OPS 的结构和 DNA 序列。O 单元在主链上由一个 d-半乳糖吡喃糖、N-乙酰-d-半乳糖胺（2-氨基-2-脱氧-d-半乳糖吡喃糖）和 d-吡喃葡萄糖组成，带有两个 3,6-二脱氧-l-木糖己吡喃糖侧链残基。这种结构与大肠杆菌 O111 和沙门氏菌 O35 非常相似。然而，这些血清型的基因簇序列与假结核耶尔森氏菌 O:10 的相似度仅为低水平，尽管基因顺序有很大的保守性。在假结核耶尔森氏菌中，O10 结构与 O:6 和 O:7 结构最为密切相关。
