Dip. di Chimica, Università di Bari, Via Orabona 4, 70126 Bari, Italy.
Bioelectrochemistry. 2009 Nov;77(1):19-25. doi: 10.1016/j.bioelechem.2009.05.002. Epub 2009 May 23.
In this work studies on rapid inhibitory interactions between heavy metals and photosynthetic materials at different organization levels were carried out by optical assay techniques, investigating the possibility of applications in the heavy metal detection field. Spinach chloroplasts, thylakoids and Photosystem II proteins were employed as biotools in combination with colorimetric assays based on dichlorophenol indophenole (DCIP) photoreduction and on fluorescence emission techniques. It was found that copper and mercury demonstrated a strong and rapid photosynthetic activity inhibition, that varied from proteins to membranes, while other metals like nickel, cobalt and manganese produced only slight inhibition effects on all tested photosynthetic materials. By emission measurements, only copper was found to rapidly influence the photosynthetic material signals. These findings give interesting information about the rapid effects of heavy metals on isolated photosynthetic samples, and are in addition to the literature data concerning the effects of growth in heavy metal enriched media.
本工作通过光学检测技术研究了重金属与不同组织水平光合作用材料之间的快速抑制相互作用，探讨了其在重金属检测领域的应用可能性。菠菜叶绿体、类囊体和 PSII 蛋白被用作生物工具，结合基于二氯酚靛酚（DCIP）光还原的比色测定法和荧光发射技术。结果表明，铜和汞对光合作用活性具有强烈和快速的抑制作用，这种抑制作用从蛋白质到膜都有所体现，而镍、钴和锰等其他金属对所有测试的光合作用材料仅产生轻微的抑制作用。通过发射测量，仅发现铜能迅速影响光合作用材料的信号。这些发现为重金属对分离的光合作用样品的快速影响提供了有趣的信息，并且除了有关在富含重金属的培养基中生长的影响的文献数据外，还提供了这些信息。
