Walsh C A, Beresford J N, Birch M A, Boothroyd B, Gallagher J A
Department of Human Anatomy and Cell Biology, University of Liverpool, England.
J Bone Miner Res. 1991 Jul;6(7):661-71. doi: 10.1002/jbmr.5650060703.
A simple technique has been developed to identify the resorption lacunae excavated by avian osteoclasts in vitro. Briefly, devitalized bovine bone wafers, with cells in situ, are fixed, stained with toluidine blue, and then examined by reflected light microscopy. Resorption pits are clearly visible using a x 10 objective, even when the resorbed areas are covered by a confluent layer of cells. The technique can be used to quantify the plan area, depth, and volume of the excavations. Depth and volume are assessed using the x 50 objective lens. This technique has been used to investigate the effects of retinyl acetate and EHDP on bone resorption.
已开发出一种简单技术，用于识别禽破骨细胞在体外挖掘出的吸收陷窝。简要地说，将带有原位细胞的失活牛骨薄片固定，用甲苯胺蓝染色，然后通过反射光显微镜检查。即使吸收区域被汇合的细胞层覆盖，使用10倍物镜也能清晰看到吸收坑。该技术可用于量化挖掘的平面面积、深度和体积。使用50倍物镜评估深度和体积。该技术已用于研究醋酸视黄酯和EHDP对骨吸收的影响。
