Okuda A, Kanehisa J, Heersche J N
Medical Research Council Group in Periodontal Physiology, Faculty of Dentistry, University of Toronto, Ontario, Canada.
J Bone Miner Res. 1990 Mar;5 Suppl 1:S115-20. doi: 10.1002/jbmr.5650051381.
It is not clear to what extent the increased bone mass observed in vertebral trabecular bone of fluoride-treated mammals is a consequence of effects of fluoride on the number and activity of osteoclasts or of osteoblasts. In the present communication, we have analyzed the effects of NaF on the activity of isolated rabbit osteoclasts cultured on thin slices of devitalized compact bovine bone. Osteoclastic resorption was quantitated by counting the number of resorption lacunae and measuring their surface area and their depth using scanning electron microscopy. Our results show that NaF in concentrations of 0.5-1.0 mM decreased the number of resorption lacunae made by individual osteoclasts and decreased the resorbed area per osteoclast. We argue that the concentration of fluoride in these experiments may be within the range "seen" by osteoclasts in mammals treated for prolonged periods with approximately 1 mg of NaF/kg body weight (bw) per day.
目前尚不清楚在接受氟化物治疗的哺乳动物的椎骨小梁骨中观察到的骨量增加，在多大程度上是氟化物对破骨细胞或成骨细胞数量和活性影响的结果。在本报告中，我们分析了氟化钠（NaF）对培养在失活致密牛骨薄片上的分离兔破骨细胞活性的影响。通过计数吸收陷窝的数量，并使用扫描电子显微镜测量其表面积和深度，对破骨细胞吸收进行定量。我们的结果表明，浓度为0.5 - 1.0 mM的NaF可减少单个破骨细胞形成的吸收陷窝数量，并减少每个破骨细胞的吸收面积。我们认为，这些实验中氟化物的浓度可能处于长期每天以约1 mg NaF/千克体重（bw）进行治疗的哺乳动物破骨细胞“所见”的范围内。
