Cheung R C, Gray C, Boyde A, Jones S J
Department of Anatomy and Developmental Biology, University College London, UK.
Bone. 1995 Jan;16(1):143-7.
Abuse of alcohol has been found to be an important risk factor for fractures and osteoporosis, and tissue culture experiments have indicated that low concentrations of ethanol can affect bone formation and resorption. This study investigated direct effects of ethanol on bone cells using an in vitro resorption assay. Osteoclasts from long bones of 19-day prehatch chicks were seeded onto slices of dentine and cultured with control medium alone, or medium containing 0.001%, 0.01% or 0.1% ethanol, at 37 degrees C with 5% CO2 for 24 h before being removed. The volumes and areas of resorption pits made in the dentine were measured using confocal laser reflection microscopy (Lasertech 1LM21 system) and the pits counted. An increase in the pit numbers and mean pit areas, volumes and volume/area ratios was observed with 0.001% and 0.01% ethanol, with a dose-related, bell-shaped curve of resorption. Greatest mean volume resorbed per pit (p < 0.05), mean area resorbed per pit (p < 0.01) and number of pits was at 0.01% ethanol. Volume/area (mean depth) per pit was greatest at 0.001% ethanol (p < 0.05). This study has shown that ethanol, even at blood concentrations experienced by the social drinker, has an immediate direct effect on bone cells in vitro, resulting in increased resorption by osteoclasts.
酒精滥用已被发现是骨折和骨质疏松症的一个重要风险因素，组织培养实验表明，低浓度乙醇会影响骨形成和骨吸收。本研究使用体外吸收试验研究乙醇对骨细胞的直接作用。将19日龄未孵化雏鸡长骨中的破骨细胞接种到牙本质切片上，分别用单独的对照培养基或含0.001%、0.01%或0.1%乙醇的培养基培养，于37℃、5%二氧化碳条件下培养24小时后取出。使用共聚焦激光反射显微镜（Lasertech 1LM21系统）测量牙本质中形成的吸收陷窝的体积和面积，并对陷窝进行计数。观察到0.001%和0.01%乙醇处理组的陷窝数量、平均陷窝面积、体积及体积/面积比增加，呈现出剂量相关的钟形吸收曲线。每个陷窝的最大平均吸收体积（p<0.05）、每个陷窝的平均吸收面积（p<0.01）和陷窝数量在0.01%乙醇处理组时最高。每个陷窝的体积/面积（平均深度）在0.001%乙醇处理组时最大（p<0.05）。本研究表明，即使是社交饮酒者血液中所达到的乙醇浓度，在体外也会对骨细胞产生直接即时效应，导致破骨细胞的吸收增加。
