Couturier Jérémy, Jacquot Jean-Pierre, Rouhier Nicolas
Interactions Arbres Microorganismes, IFR 110 Génomique Ecophysiologie et Ecologie Fonctionnelles, Unité Mixte de Recherches 1136 INRA-Nancy Université, 54506 Vandoeuvre-lès-Nancy Cedex, France.
Cell Mol Life Sci. 2009 Aug;66(15):2539-57. doi: 10.1007/s00018-009-0054-y. Epub 2009 Jun 9.
The genome sequencing of prokaryotic and eukaryotic photosynthetic organisms enables a comparative genomic study of the glutaredoxin (Grx) family. The analysis of 58 genomes, using a specific motif composed of the active site sequence and of amino acids involved in glutathione binding, led to an updated classification of Grxs into six classes. Only two classes (I and II) are common to all photosynthetic organisms. Eukaryotes and cyanobacteria have two specific Grx classes (classes III and IV and classes V and VI, respectively). The classes IV, V and VI have not yet been identified and contain multimodular Grx fusions. In addition, putative Grx partners were identified from the presence of fusion proteins, the conservation of gene order in bacterial operons, and the gene co-occurrence. The genes encoding class II Grxs and BolA/YrbA proteins are frequently adjacent, in the same transcriptional orientation in prokaryote genomes and present in the same organisms.
对原核生物和真核生物光合生物体进行基因组测序，有助于对谷氧还蛋白（Grx）家族开展比较基因组研究。利用由活性位点序列和参与谷胱甘肽结合的氨基酸组成的特定基序，对58个基因组进行分析，从而将Grx更新分类为六个类别。所有光合生物体都共有仅两个类别（I和II）。真核生物和蓝细菌分别有两个特定的Grx类别（III和IV类以及V和VI类）。IV、V和VI类尚未得到鉴定，且包含多模块Grx融合体。此外，通过融合蛋白的存在、细菌操纵子中基因顺序的保守性以及基因共现情况，鉴定出了假定的Grx伙伴。编码II类Grx和BolA/YrbA蛋白的基因常常相邻，在原核生物基因组中具有相同的转录方向，且存在于相同的生物体中。
