Liu Yanshuang, Han Xia, Yu Juanjuan, Li Yueyue, Sun Meihong, Pang Qiuying, Li Ying, Dai Shaojun
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, 150040, China.
Development Center of Plant Germplasm Resources, College of Life Sciences, Shanghai Normal University, Shanghai, 200234, China.
BMC Plant Biol. 2025 May 8;25(1):605. doi: 10.1186/s12870-025-06547-1.
Glutaredoxins (GRX) are key oxidoreductases that modulate protein redox states during plant development and stress responses. Alkaligrass (Puccinellia tenuiflora) is a highly salt-tolerant forage grass, but its GRX gene family (PutGRXs) remains uncharacterized, unlike those in Arabidopsis and other plants.
We identified 25 PutGRX genes in the P. tenuiflora genome. Phylogenetic analysis revealed close evolutionary ties to monocotyledonous rice (Oryza sativa). Based on gene structure and conserved domains, PutGRXs were classified into three groups: five CGFS-type, eleven CPYC-type, and nine CC-type GRXs. Promoter analysis identified numerous cis-acting elements linked to abiotic stresses (e.g., light, drought, heat, cold) and hormone responses, suggesting a pivotal role in stress adaptation. Tissue-specific expression profiling showed differential PutGRX expression in roots, leaves, stems, flowers, and sheaths, with most genes responding to NaCl, NaHCO, and NaCO stresses. Functional characterization of chloroplast-localized PutGrxS12 demonstrated its importance in plant growth and ROS scavenging under salinity stress.
This study offers the first comprehensive genomic and functional analysis of the PutGRX family in P. tenuiflora, highlighting its conservation, classification, and stress-responsive roles. Our findings advance understanding of GRX-mediated stress tolerance and provide potential targets for engineering salt-resistant crops.
谷氧还蛋白（GRX）是关键的氧化还原酶，在植物发育和应激反应过程中调节蛋白质的氧化还原状态。碱茅（Puccinellia tenuiflora）是一种高度耐盐的牧草，但其GRX基因家族（PutGRXs）仍未得到表征，这与拟南芥和其他植物不同。
我们在碱茅基因组中鉴定出25个PutGRX基因。系统发育分析表明，它们与单子叶水稻（Oryza sativa）有着密切的进化关系。根据基因结构和保守结构域，PutGRXs被分为三组：五个CGFS型、十一个CPYC型和九个CC型GRX。启动子分析确定了许多与非生物胁迫（如光照、干旱、高温、低温）和激素反应相关的顺式作用元件，表明其在胁迫适应中起关键作用。组织特异性表达谱显示，PutGRX在根、叶、茎、花和叶鞘中的表达存在差异，大多数基因对NaCl、NaHCO₃和Na₂CO₃胁迫有反应。叶绿体定位的PutGrxS12的功能表征表明，它在盐胁迫下对植物生长和活性氧清除具有重要作用。
本研究首次对碱茅中的PutGRX家族进行了全面的基因组和功能分析，突出了其保守性、分类和应激反应作用。我们的研究结果推进了对GRX介导的胁迫耐受性的理解，并为培育抗盐作物提供了潜在靶点。
