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小鼠乳腺肿瘤病毒特异性mRNA的鉴定

Identification of mouse mammary tumor virus-specific mRNA.

作者信息

Groner B, Hynes N E, Diggelmann H

出版信息

J Virol. 1979 Apr;30(1):417-20. doi: 10.1128/JVI.30.1.417-420.1979.

DOI:10.1128/JVI.30.1.417-420.1979
PMID:225528
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC353340/
Abstract

Complementary DNA corresponding to the RNA genome of mouse mammary tumor virus was used to identify viral RNA contained in polysomes of a virus-producing mammary tumor cell line. Separation of polysomal mRNA by agarose gel electrophoresis, transfer of the RNA to diazobenzyloxymethyl paper, and hybridization with 32P-labeled mouse mammary tumor virus complementary DNA revealed three viral RNA size classes of 10, 8.8, and 4.4 kilobases in length, respectively.

摘要

与小鼠乳腺肿瘤病毒RNA基因组相对应的互补DNA被用于鉴定病毒产生的乳腺肿瘤细胞系多核糖体中所含的病毒RNA。通过琼脂糖凝胶电泳分离多核糖体mRNA，将RNA转移至重氮苄氧基甲基纸上，并与32P标记的小鼠乳腺肿瘤病毒互补DNA杂交，结果显示分别有长度为10、8.8和4.4千碱基的三种病毒RNA大小类别。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8eb/353340/6f3cbb4d8cbb/jvirol00184-0429-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8eb/353340/6f3cbb4d8cbb/jvirol00184-0429-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8eb/353340/6f3cbb4d8cbb/jvirol00184-0429-a.jpg

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1
Identification of mouse mammary tumor virus-specific mRNA.小鼠乳腺肿瘤病毒特异性mRNA的鉴定
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引用本文的文献

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Sequence and expression of the mouse mammary tumour virus env gene.小鼠乳腺肿瘤病毒env基因的序列与表达
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2
Dexamethasone-stimulated expression of a proviral copy of mouse mammary tumor virus env mRNA.地塞米松刺激小鼠乳腺肿瘤病毒env mRNA原病毒拷贝的表达。
J Virol. 1984 May;50(2):632-5. doi: 10.1128/JVI.50.2.632-635.1984.
3
Cloned mouse mammary tumor virus DNA exhibits glucocorticoid-dependent expression in simian virus 40-transformed mink cells.克隆的小鼠乳腺肿瘤病毒DNA在猿猴病毒40转化的貂细胞中表现出糖皮质激素依赖性表达。

本文引用的文献

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The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation.通过聚丙烯酰胺凝胶电泳测定核糖核酸的分子量。构象变化的影响。
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Hormone-responsive expression of an endogenous proviral gene of mouse mammary tumor virus after molecular cloning and gene transfer into cultured cells.小鼠乳腺肿瘤病毒内源性前病毒基因在分子克隆并转入培养细胞后受激素应答的表达
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2038-42. doi: 10.1073/pnas.78.4.2038.
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Protein-coding potential of mouse mammary tumor virus genome RNA as examined by in vitro translation.通过体外翻译检测小鼠乳腺肿瘤病毒基因组RNA的蛋白质编码潜能。
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Structure and processing of the mouse mammary tumor virus glycoprotein precursor pr73env.小鼠乳腺肿瘤病毒糖蛋白前体pr73env的结构与加工
J Virol. 1980 Aug;35(2):349-61. doi: 10.1128/JVI.35.2.349-361.1980.
在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
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mRNA complexity and egg white protein mRNA content in mature and hormone-withdrawn oviduct.成熟和激素撤除后的输卵管中mRNA复杂性及蛋清蛋白mRNA含量
Cell. 1977 Aug;11(4):923-32. doi: 10.1016/0092-8674(77)90303-8.
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Isolation of specific messenger RNA by adsorption of polysomes to matrix-bound antibody.通过多核糖体吸附到基质结合抗体上来分离特定信使核糖核酸。
Nucleic Acids Res. 1977 Jan;4(1):71-84. doi: 10.1093/nar/4.1.71.
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Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.通过转移至重氮苄氧基甲基纸并与DNA探针杂交来检测琼脂糖凝胶中特定RNA的方法。
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Immunoadsorption of specific chicken oviduct polysomes. Isolation of ovalbumin, ovomucoid, and lysozyme messenger RNA.特异性鸡输卵管多核糖体的免疫吸附。卵清蛋白、卵类粘蛋白和溶菌酶信使核糖核酸的分离。
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Size and genetic content of viral RNAs in avian oncovirus-infected cells.禽肿瘤病毒感染细胞中病毒RNA的大小和遗传内容。
J Virol. 1977 Oct;24(1):47-63. doi: 10.1128/JVI.24.1.47-63.1977.