转运RNA与小鼠乳腺肿瘤病毒中RNA指导的DNA合成的引发

J Virol. 1980 Jul;35(1):31-40. doi: 10.1128/JVI.35.1.31-40.1980.

The low-molecular-weight RNAs of mouse mammary tumor virus (MuMTV) were examined by two-dimensional acrylamide gel electrophoresis. Unlike other retroviruses, MuMTV was found to contain only two major fractions of tRNA. These have been fully characterized and shown to correspond to the published sequences for tRNA1+2Lys and tRNA3Lys. By determining which of these tRNA's was most tightly associated with the MuMTV genome and which of them acquired label from [alpha-32P]deoxynucleoside triphosphates in limited DNA synthesis reactions, we identified tRNA3Lys as the primer for MuMTV reverse transcription in vitro. tRNA3Lys does not share any unusual sequence feature with the other previously characterized retrovirus primers, tRNATrp and tRNAPro.

通过二维丙烯酰胺凝胶电泳对小鼠乳腺肿瘤病毒（MuMTV）的低分子量RNA进行了检测。与其他逆转录病毒不同，发现MuMTV仅包含两个主要的tRNA组分。这些组分已得到充分表征，并显示与已发表的tRNA1+2Lys和tRNA3Lys序列相对应。通过确定这些tRNA中哪一个与MuMTV基因组结合最紧密，以及在有限的DNA合成反应中哪一个从[α-32P]脱氧核苷三磷酸获得标记，我们确定tRNA3Lys是MuMTV体外逆转录的引物。tRNA3Lys与其他先前表征的逆转录病毒引物tRNATrp和tRNAPro没有任何异常的序列特征。