Kong Chang-Suk, Kim Jung-Ae, Kim Se-Kwon
Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Republic of Korea.
Food Chem Toxicol. 2009 Oct;47(10):2401-6. doi: 10.1016/j.fct.2009.06.010. Epub 2009 Jun 9.
In this study, we investigated the effect of sulfated glucosamine (SGlc) on adipogenesis of 3T3-L1 adipocytes during differentiation of preadipocytes into adipocytes by measuring lipid accumulation and adipogenesis related factors. Treatment with SGlc reduced the triglyceride content in Oil-Red O staining and enhanced glycerol secretion in adipocytes in a dose-dependent manner. In addition, SGlc induced the down-regulation of adipogenesis related factors and adipocyte specific gene promoters. Moreover, treatment of 3T3-L1 adipocytes with SGlc activated the phosphorylated adenosine monophosphate-activated protein kinase (AMPK) alpha and beta along with their substrate, acetyl-CoA carboxylase (ACC). These results suggest that inhibitory effect of SGlc on adipocyte differentiation might be mediated through the up-regulation of AMPK pathway.
在本研究中，我们通过测量脂质积累和脂肪生成相关因子，研究了硫酸化葡萄糖胺（SGlc）在前脂肪细胞分化为脂肪细胞过程中对3T3-L1脂肪细胞脂肪生成的影响。SGlc处理以剂量依赖性方式降低了油红O染色中的甘油三酯含量，并增强了脂肪细胞中的甘油分泌。此外，SGlc诱导脂肪生成相关因子和脂肪细胞特异性基因启动子的下调。此外，用SGlc处理3T3-L1脂肪细胞可激活磷酸化的腺苷单磷酸激活蛋白激酶（AMPK）α和β及其底物乙酰辅酶A羧化酶（ACC）。这些结果表明，SGlc对脂肪细胞分化的抑制作用可能是通过AMPK途径的上调介导的。
