Tettelin Hervé, Feldblyum Tamara
Institute for Genome Sciences, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, USA.
Methods Mol Biol. 2009;551:231-47. doi: 10.1007/978-1-60327-999-4_18.
For over 30 yr, the Sanger method has been the standard for DNA sequencing. Instruments have been developed and improved over time to increase throughput, but they always relied on the same technology. Today, we are facing a revolution in DNA sequencing with many drastically different platforms that have become or will soon become available on the market. We review a number of sequencing technologies and provide examples of applications. We also discuss the impact genomics and new DNA sequencing approaches have had on various fields of biological research.
30多年来，桑格法一直是DNA测序的标准方法。随着时间的推移，仪器不断得到开发和改进以提高通量，但它们始终依赖于相同的技术。如今，我们正面临DNA测序领域的一场革命，许多截然不同的平台已经或即将投放市场。我们回顾了多种测序技术并提供了应用实例。我们还讨论了基因组学和新的DNA测序方法对生物研究各个领域所产生的影响。
