Maiden M C
The Wellcome Trust Centre for the Epidemiology of Infectious Disease, Department of Zoology, University of Oxford, United Kingdom.
Int J Med Microbiol. 2000 May;290(2):183-90. doi: 10.1016/S1438-4221(00)80089-2.
High-throughput nucleotide sequence determination technologies present new opportunities for studies of bacterial pathogens by enabling the accumulation of large volumes of biodiversity information from isolate collections. Population studies, which combine these data with epidemiological, phylogenetic, and evolutionary concepts, provide insights into the behaviour of pathogens that are unavailable from other approaches as they address questions of relevance to pathogenesis from the perspective of the infectious organism rather from that of the host. Hypothesis-driven analyses applied to these data permit the determination of microbial population diversity and structure, the identification of the mechanisms of genetic change in bacterial populations, and the generation of models of pathogen evolution. The nucleotide sequence-based population studies performed to date demonstrate a spectrum of nucleotide sequence diversity, population structure, and evolutionary mechanisms among pathogenic bacteria. The rapid development of nucleotide sequence determination and analysis techniques provides the tools necessary for the prosecution of population studies on an increasing number of bacterial pathogens.
高通量核苷酸序列测定技术通过从分离株库中积累大量生物多样性信息，为细菌病原体研究带来了新机遇。群体研究将这些数据与流行病学、系统发育学和进化概念相结合，从感染生物体而非宿主的角度探讨与发病机制相关的问题，从而提供了其他方法无法获得的病原体行为见解。对这些数据进行假设驱动的分析，可以确定微生物群体的多样性和结构，识别细菌群体中基因变化的机制，并生成病原体进化模型。迄今为止进行的基于核苷酸序列的群体研究展示了病原菌之间核苷酸序列多样性、群体结构和进化机制的范围。核苷酸序列测定和分析技术的快速发展为越来越多的细菌病原体进行群体研究提供了必要的工具。
