Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 151921, South Korea.
Reproduction. 2009 Sep;138(3):483-92. doi: 10.1530/REP-09-0163. Epub 2009 Jun 12.
Synaptonemal complexes (SCs) are associated with synapsis of homologous chromosomes, chiasmata distribution, recombination and segregation of chromosomes during the extended prophase of meiosis I. Three isoforms of SC proteins, SYCP1, SYCP2 and SYCP3, were identified as the structural proteins of SCs, and may be involved in the assembly and disassembly of SCs. The aim of this present study is to determine the pattern of expression of chicken homologues of SYCP family members during ovarian and testicular development. Protein sequence analysis using CLUSTAL X revealed that the sequences and potential phosphorylation sites of chicken SYCP family proteins were highly conserved with mammalian homologues of SYCP family proteins. Quantitative real-time-PCR and in situ hybridisation analysis revealed that chicken SYCP family members were differentially expressed during ovarian and testicular development. During ovarian development, all chicken SYCP family members were detected in primordial germ cells (PGCs) until embryonic day (E) 8.0; the expression continued in proliferating pre-meiotic oogonia until E15.5 and was upregulated in meiotic prophase I oocytes until hatching. After hatching, all chicken SYCP family members were detected at a low level until 24-weeks-old. During testicular development, all chicken SYCP family members were detected in PGCs until E13.0; the expression continued in pro-spermatogonia and proliferating spermatogonia for up to 8 weeks, and was upregulated in meiotic prophase I spermatocytes in adults. Our data demonstrate the expression pattern of meiosis associated SYCP family members during ovarian and testicular development in chickens.
联会复合体（SCs）与同源染色体的联会、交叉分布、染色体的重组和减数分裂 I 前期的分离有关。SYCP1、SYCP2 和 SYCP3 三种 SC 蛋白异构体被鉴定为 SC 的结构蛋白，可能参与 SC 的组装和拆卸。本研究旨在确定鸡 SYCP 家族成员在卵巢和睾丸发育过程中的表达模式。使用 CLUSTAL X 进行的蛋白质序列分析表明，鸡 SYCP 家族蛋白的序列和潜在磷酸化位点与哺乳动物 SYCP 家族蛋白的同源物高度保守。定量实时 PCR 和原位杂交分析显示，鸡 SYCP 家族成员在卵巢和睾丸发育过程中存在差异表达。在卵巢发育过程中，所有鸡 SYCP 家族成员都在原始生殖细胞（PGCs）中检测到，直到胚胎日（E）8.0；在增殖的减数前期卵母细胞中持续表达，直到孵化时上调。孵化后，所有鸡 SYCP 家族成员的表达水平一直较低，直到 24 周龄。在睾丸发育过程中，所有鸡 SYCP 家族成员都在 PGC 中检测到，直到 E13.0；在原精原细胞和增殖精原细胞中持续表达长达 8 周，在成年减数分裂 I 前期精母细胞中上调。我们的数据表明，鸡在卵巢和睾丸发育过程中与减数分裂相关的 SYCP 家族成员的表达模式。
