WCU Biomodulation Major, Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Korea.
Theriogenology. 2010 Sep 15;74(5):765-76. doi: 10.1016/j.theriogenology.2010.04.001. Epub 2010 May 26.
The autosomal gene deleted in azoospermia-like (DAZL), which was identified as a member of the deleted in azoospermia (DAZ) family, is homologous to the Drosophila gene BOULE. The authors investigated the sequence similarities of chicken DAZL (cDAZL) with several invertebrate and vertebrate DAZL proteins using CLUSTAL X. A comparison of the primary sequence of cDAZL with other DAZL proteins indicated significant similarities: 70-82% with reptiles, 63-68% with mammals, 51-67% with amphibians, and 42-49% with fishes. The conserved expression pattern of cDAZL was examined by reverse transcription-PCR, quantitative real-time PCR, and in situ hybridization during primordial germ cell (PGC) settlement in the gonads and germ-line development. Among several tissues examined on embryonic day E6.5, DAZL expression was detected specifically in male and female gonads. Quantitative real-time PCR and in situ hybridization revealed strong cDAZL expression in PGCs. When the PGCs differentiated into germ cells, cDAZL expression was slightly decreased; however, expression was continuously detected in germ-line cells until the adult stage. We inferred that cDAZL expression was conserved in PGCs and during germ-line differentiation until the adult stage, making them a valuable molecular marker for studies of PGC differentiation and germ-line development in chickens.
常染色体基因缺失在无精子症样（DAZL）中，被鉴定为缺失在无精子症（DAZ）家族的一员，与果蝇基因 BOULE 同源。作者使用 CLUSTAL X 研究了鸡 DAZL（cDAZL）与几种无脊椎动物和脊椎动物 DAZL 蛋白的序列相似性。与其他 DAZL 蛋白的一级序列比较表明，cDAZL 具有显著的相似性：与爬行动物的相似度为 70-82%，与哺乳动物的相似度为 63-68%，与两栖动物的相似度为 51-67%，与鱼类的相似度为 42-49%。通过逆转录-PCR、定量实时 PCR 和原位杂交在性腺中原始生殖细胞（PGC）定居和生殖系发育过程中检查了 cDAZL 的保守表达模式。在胚胎第 6.5 天检查的几种组织中，DAZL 表达特异性地在雄性和雌性性腺中检测到。定量实时 PCR 和原位杂交显示 PGC 中存在强烈的 cDAZL 表达。当 PGC 分化为生殖细胞时，cDAZL 表达略有下降；然而，在生殖系细胞中持续检测到表达，直到成年阶段。我们推断，cDAZL 表达在 PGC 中以及在生殖系分化过程中是保守的，直到成年阶段，这使它们成为研究鸡 PGC 分化和生殖系发育的有价值的分子标记。
