Park Yongkeun, Popescu Gabriel, Badizadegan Kamran, Dasari Ramachandra R, Feld Michael S
Opt Express. 2006 Sep 4;14(18):8263-8. doi: 10.1364/oe.14.008263.
We have developed diffraction phase and fluorescence (DPF) microscopy as a new technique for simultaneous quantitative phase imaging and epi-fluorescence investigation of live cells. The DPF instrument consists of an interference microscope, which is incorporated into a conventional inverted fluorescence microscope. The quantitative phase images are characterized by sub-nanometer optical path-length stability over periods from milliseconds to a cell lifetime. The potential of the technique for quantifying rapid nanoscale motions in live cells is demonstrated by experiments on red blood cells, while the composite phase-fluorescence imaging mode is exemplified with mitotic kidney cells.
我们已经开发出衍射相和荧光（DPF）显微镜技术，用于对活细胞同时进行定量相成像和落射荧光研究。DPF仪器由一台干涉显微镜组成，该干涉显微镜被整合到传统的倒置荧光显微镜中。定量相图像的特点是在从毫秒到细胞寿命的时间段内具有亚纳米级的光程长度稳定性。通过对红细胞的实验证明了该技术在量化活细胞中快速纳米级运动方面的潜力，而有丝分裂的肾细胞则例证了复合相-荧光成像模式。
