Ding Huafeng, Popescu Gabriel
Quantitative Light Imaging Laboratory, Department of Electrical and Computer Engineering, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Opt Express. 2010 Jan 18;18(2):1569-75. doi: 10.1364/OE.18.001569.
We present Instantaneous Spatial Light Interference Microscopy (iSLIM) as a new quantitative phase method that combines the benefits of white light illumination in Zernike's phase contrast microscopy and phase stability associated diffraction phase microscopy. iSLIM is implemented as an add-on module to a commercial phase contrast microscope, and enables new features to quantitative phase imaging: diminished speckle effects due to white light illumination, multimodal investigation potential due to overlaying with other modalities of the microscope (e.g. fluorescence, DIC, phase contrast), and spectroscopic potential due to the broad band light. We show proof of principle results by multicolor phase imaging of microsphere and red blood cells, and dynamic imaging of nanoscale cell membrane fluctuations.
我们提出了瞬时光学空间干涉显微镜（iSLIM），这是一种新的定量相位方法，它结合了泽尼克相衬显微镜中白光照明的优点以及与衍射相显微镜相关的相位稳定性。iSLIM被实现为商业相衬显微镜的一个附加模块，并为定量相位成像带来了新特性：由于白光照明减少了散斑效应，由于与显微镜的其他模态（如荧光、微分干涉对比、相衬）叠加而具有多模态研究潜力，以及由于宽带光而具有光谱潜力。我们通过微球和红细胞的多色相位成像以及纳米级细胞膜波动的动态成像展示了原理验证结果。
