Stomeo Francesca, Portillo Maria C, Gonzalez Juan M
Departament: Geoecología, Biogeoquímica y Microbiología Ambiental, Instituto de Recursos Naturales y Agrobiología, IRNAS-CSIC, Avda. Reina Mercedes 10, 41012, Sevilla, Spain.
Curr Microbiol. 2009 Sep;59(3):321-5. doi: 10.1007/s00284-009-9437-4. Epub 2009 Jun 18.
The most representative bacterium (Pseudonocardia sp.) and fungus (Fusarium sp.) from the microbial communities of a cave containing paleolithic paintings were isolated and their growth on natural substrates assessed. Growth was tested at the in situ and optimal, laboratory growth temperature. Development was analyzed with and without supplemented nutrients (glucose, ammonium, phosphate, peptone). Results showed that the assayed bacterium on natural substrate was able to develop best at in situ temperature and the addition of organic nutrients and/or phosphate enhanced its growth. The growth of the assayed fungus, however, was limited by low temperature and the availability of ammonium. These results confirm a differential behavior of microorganisms between the laboratory and the natural environments and could explain previous invasion of fungi reported for some caves with prehistoric paintings.
从一个有旧石器时代壁画的洞穴微生物群落中分离出最具代表性的细菌（诺卡氏菌属）和真菌（镰刀菌属），并评估它们在天然基质上的生长情况。在原位温度和最佳实验室生长温度下测试生长情况。在添加和不添加营养物质（葡萄糖、铵、磷酸盐、蛋白胨）的情况下分析其生长发育情况。结果表明，所检测的细菌在天然基质上能够在原位温度下生长最佳，添加有机营养物质和/或磷酸盐可促进其生长。然而，所检测真菌的生长受到低温和铵可用性的限制。这些结果证实了微生物在实验室环境和自然环境之间的差异行为，并可以解释先前报道的一些有史前壁画的洞穴中真菌的入侵情况。
