一种用于评估 5'-核苷酸抗氧化活性的流动注射化学发光法。

A flow-injection chemiluminescent method for the evaluation of the antioxidant activity of 5'-nucleotides.

机构信息

State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

出版信息

Luminescence. 2010 Jul-Aug;25(4):300-6. doi: 10.1002/bio.1149.

DOI:10.1002/bio.1149

PMID:19536768

Abstract

In the present work, a simple chemiluminescence (CL) method coupled with flow-injection analysis for the evaluation of antioxidant activity of 5'-nucleotides (5'-AMP, 5'-CMP, 5'-GMP, 5'-UMP) was proposed. It is based on inhibition effect of the studied substances on CL emission of luminol-potassium ferricyanide-pyrogallol. Experiments were performed to evaluate the nature of the inhibition by 5'-nucleotides of the CL reaction and their antioxidant activities. Based on the experimental results, it was observed that 5'-nucleotides are available antioxidants that could effectively scavenge superoxide anion free radicals in a concentration-dependent way. This will provide a basis for further development of the use of nucleotides.

摘要

在本工作中，提出了一种简单的化学发光（CL）方法，并结合流动注射分析来评估 5'-核苷酸（5'-AMP、5'-CMP、5'-GMP、5'-UMP）的抗氧化活性。它基于被研究物质对鲁米诺-铁氰化钾-没食子酸的 CL 发射的抑制作用。进行了实验以评估 5'-核苷酸对 CL 反应的抑制性质及其抗氧化活性。根据实验结果，观察到 5'-核苷酸是可用的抗氧化剂，它们可以有效地清除超氧阴离子自由基，且这种清除作用具有浓度依赖性。这将为进一步开发核苷酸的应用提供依据。