Kesarcodi-Watson A, Kaspar H, Lategan M J, Gibson L
Cawthron Institute, Nelson, New Zealand.
J Fish Dis. 2009 Jun;32(6):499-507. doi: 10.1111/j.1365-2761.2009.01006.x. Epub 2008 Apr 12.
Bacterial pathogens of Greenshell mussel (GSM) larvae can cause batch losses during hatchery production. Twenty-two isolates were screened using a larval bioassay. Two strains were identified as potential pathogens. Phenotypic identification of these strains revealed two non-reactive Gram-negative, oxidase positive rods. Sequencing of the 16S rRNA gene identified Vibrio splendidus and a V. coralliilyticus/neptunius-like isolate as pathogens of GSM larvae, with an ability to cause 83% and 75% larval mortality in vitro, respectively, at a concentration of 10(2) CFU mL(-1). Histopathology indicated that the route of infection was via the digestive system. Using healthy larvae as target hosts, Koch's postulates were confirmed for the two isolates. This is the first report on pathogens of GSM larvae.
绿壳贻贝（GSM）幼虫的细菌病原体可在孵化场生产过程中导致批量损失。使用幼虫生物测定法筛选了22株分离株。鉴定出两株为潜在病原体。这些菌株的表型鉴定显示为两株无反应性的革兰氏阴性、氧化酶阳性杆菌。16S rRNA基因测序鉴定出灿烂弧菌和一株溶珊瑚弧菌/海神弧菌样分离株为GSM幼虫的病原体，在浓度为10(2) CFU mL(-1)时，它们在体外分别能导致83%和75%的幼虫死亡率。组织病理学表明感染途径是通过消化系统。以健康幼虫作为目标宿主，对这两株分离株证实了科赫法则。这是关于GSM幼虫病原体的首次报道。
