采用微孔板的新型紫外分光光度法测定多酚含量的测定条件和验证。
Assay conditions and validation of a new UV spectrophotometric method using microplates for the determination of polyphenol content.
机构信息
Department of Pharmacy and Pharmaceutical Technology, School of Pharmacy, University of Navarra, Pamplona (Navarra), Spain.
出版信息
Fitoterapia. 2009 Dec;80(8):465-7. doi: 10.1016/j.fitote.2009.06.008. Epub 2009 Jun 21.
PMID:19540907
Abstract
A new spectrophotometric assay for the quantification of polyphenolic content has been validated. It is based on Prussian Blue method and adapted to microplate spectrophotometry. Prussian Blue reaction is critically dependent on reaction time, but microplate spectrophotometry permits exact measures of many samples at the same time. This new method is precise, reproducible, repeatable and exact.
摘要
已验证一种用于定量测定多酚含量的新分光光度测定法。它基于普鲁士蓝法，并适应于微孔板分光光度法。普鲁士蓝反应严重依赖于反应时间，但微孔板分光光度法允许同时精确测量许多样品。这种新方法精确、可重现、可重复且准确。
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