Martin C, Alonso M I, Santiago C, Moro J A, De la Mano A, Carretero R, Gato A
Departamento de Anatomía y Radiología, Laboratorio de Desarrollo y Teratología del Sistema Nervioso, Instituto de Neurociencias de Castilla y León, Facultad de Medicina, Universidad de Valladolid, Valladolid, Spain.
Int J Dev Neurosci. 2009 Nov;27(7):733-40. doi: 10.1016/j.ijdevneu.2009.06.002. Epub 2009 Jun 21.
Cerebrospinal fluid has shown itself to be an essential brain component during development. This is particularly evident at the earliest stages of development where a lot of research, performed mainly in chick embryos, supports the evidence that cerebrospinal fluid is involved in different mechanisms controlling brain growth and morphogenesis, by exerting a trophic effect on neuroepithelial precursor cells (NPC) involved in controlling the behaviour of these cells. Despite it being known that cerebrospinal fluid in mammals is directly involved in corticogenesis at fetal stages, the influence of cerebrospinal fluid on the activity of NPC at the earliest stages of brain development has not been demonstrated. Here, using "in vitro" organotypic cultures of rat embryo brain neuroepithelium in order to expose NPC to or deprive them of cerebrospinal fluid, we show that the neuroepithelium needs the trophic influence of cerebrospinal fluid to undergo normal rates of cell survival, replication and neurogenesis, suggesting that NPC are not self-sufficient to induce their normal activity. This data shows that cerebrospinal fluid is an essential component in chick and rat early brain development, suggesting that its influence could be constant in higher vertebrates.
脑脊液已证明自身在发育过程中是大脑的重要组成部分。这在发育的最早阶段尤为明显，许多主要在鸡胚中进行的研究支持了以下证据：脑脊液通过对参与控制这些细胞行为的神经上皮前体细胞（NPC）施加营养作用，参与控制大脑生长和形态发生的不同机制。尽管已知哺乳动物的脑脊液在胎儿期直接参与皮质发生，但脑脊液在大脑发育最早阶段对NPC活性的影响尚未得到证实。在这里，我们使用大鼠胚胎脑神经上皮的“体外”器官型培养物，以使NPC暴露于脑脊液或使其脱离脑脊液，结果表明神经上皮需要脑脊液的营养影响才能经历正常的细胞存活、复制和神经发生速率，这表明NPC不足以自主诱导其正常活性。该数据表明脑脊液是鸡和大鼠早期大脑发育的重要组成部分，这表明其影响在高等脊椎动物中可能是持续存在的。
