Salmon S, Mazière J C, Santus R, Morlière P
Muséum National d'Histoire Naturelle, Laboratoire de Physico-chimie de l'Adaptation Biologique, INSERM U312, Paris, France.
Biochim Biophys Acta. 1991 Oct 15;1086(1):1-6. doi: 10.1016/0005-2760(91)90147-a.
The tryptophan residues of HDL are important chromophores of the interstitial fluid feeding the epidermal cells. The UVB light readily photoionizes tryptophan residues as demonstrated by the increase in their photolysis yield in presence of N2O. Saturation of the HDL solution with a N2O/O2 mixture (80%:20%, v/v) decreases the peroxidation of HDL lipids, thereby implying that lipid peroxidation is at least partly induced by tryptophan photolysis. Addition of EDTA and desferrioxamine to HDL solutions loaded with either Cu2+ or Fe2+ ions suggests that Fe2+ could be the 'contaminating' trace metal ions that are required to explain the occurrence of lipid photoperoxidation induced by photons absorbed by tryptophan residues.
高密度脂蛋白（HDL）中的色氨酸残基是为表皮细胞提供营养的组织液的重要发色团。如在一氧化二氮（N2O）存在的情况下其光解产率增加所证明的，中波紫外线（UVB）光很容易使色氨酸残基发生光离子化。用N2O/O2混合物（80%:20%，v/v）使HDL溶液饱和可降低HDL脂质的过氧化，从而意味着脂质过氧化至少部分是由色氨酸光解诱导的。向负载有Cu2+或Fe2+离子的HDL溶液中添加乙二胺四乙酸（EDTA）和去铁胺表明，Fe2+可能是“污染性”痕量金属离子，需要用它们来解释由色氨酸残基吸收的光子诱导的脂质光过氧化的发生。
