Salmon S, Haigle J, Bazin M, Santus R, Mazière J C, Dubertret L
Muséum National d'Histoire Naturelle, Laboratoire de Photobiologie, INSERM U312, Paris, France.
J Photochem Photobiol B. 1996 May;33(3):233-8. doi: 10.1016/1011-1344(95)07260-8.
Suction blister fluid is a good representative of the interstitial fluid feeding the epidermal cells. Lipoproteins contained in the suction blister fluid of healthy volunteers are readily photo-oxidized by UV radiation in the wavelength range 290-385 nm. Absorbed light doses equivalent to one minimal erythemal dose absorbed by skin exposed to UVB (e.g. 290-320 nm) are sufficient to induce lipid peroxidation and modification of apolipoproteins A-I, A-II and B. Albumin, which is known to protect serum fully from oxidative stresses, is not so effective against photo-oxidation. Although tryptophan (Trp) residues of proteins contained in the suction blister fluid are photo-oxidized, apolipoprotein A-II, which does not contain any chromophoric Trp residue, is also altered by the UV radiation. With regard to results obtained with a model reconstituted fluid, it is suggested that the radical chain reaction of the lipid peroxidation can transfer the initial photodamage at sites not directly susceptible to photo-oxidation. The biological implications of these results are discussed.
抽吸水疱液是滋养表皮细胞的组织液的良好代表。健康志愿者抽吸水疱液中所含的脂蛋白很容易被波长范围在290 - 385 nm的紫外线辐射光氧化。相当于皮肤暴露于UVB（如290 - 320 nm）所吸收的一个最小红斑剂量的吸收光剂量足以诱导脂质过氧化以及载脂蛋白A-I、A-II和B的修饰。已知能完全保护血清免受氧化应激的白蛋白，对光氧化的作用并不那么有效。尽管抽吸水疱液中所含蛋白质的色氨酸（Trp）残基会被光氧化，但不含任何发色Trp残基的载脂蛋白A-II也会因紫外线辐射而改变。关于用模型重构液获得的结果表明，脂质过氧化的自由基链反应可以将初始光损伤转移到不易直接发生光氧化的部位。讨论了这些结果的生物学意义。
