Mäder M, Retzlaff K, Felgenhauer K
Neurologische Klinik, Universität Göttingen.
Eur J Clin Chem Clin Biochem. 1991 Aug;29(8):481-5. doi: 10.1515/cclm.1991.29.8.481.
Patterns of highly glycosylated proteins with mainly cationic isoelectric points, pH 6.5-9.5, were observed in serum and cerebrospinal fluid of patients with various disorders. Detection was performed after isoelectric focusing, using an immunoassay specific for digoxigenylated carbohydrate moieties of glycoconjugates. To our knowledge, these glycoproteins have not hitherto been described as regular serum proteins. The patterns were found among 7% of the patients studied (n = 400). Similar bands were not detectable in a reference group of 100 persons without clinical symptoms. The glycoprotein pattern was specific for each individual. The pathophysiological meaning of these glycoproteins as well as the basic biochemistry has not yet been evaluated. The glycoproteins, however, were shown to differ from immunoglobulin G, oligoclonal immunoglobulin G, paraprotein or from regular cationic serum protein. By comparison with standard glycoproteins a carbohydrate content of 30 +/- 10% was roughly suggested. The oligosaccharides contain probably sialic acid as evidenced by lectin binding. Although the diagnoses varied, 90% of the patients with this glycoprotein pattern had inflammatory processes.
在患有各种疾病的患者的血清和脑脊液中观察到了主要具有阳离子等电点（pH 6.5 - 9.5）的高度糖基化蛋白质模式。在等电聚焦后，使用针对糖缀合物中洋地黄毒苷化碳水化合物部分的免疫测定法进行检测。据我们所知，这些糖蛋白迄今尚未被描述为常规血清蛋白。在所研究的7%的患者（n = 400）中发现了这种模式。在100名无临床症状的参考组中未检测到类似条带。糖蛋白模式对每个个体都是特异的。这些糖蛋白的病理生理意义以及基础生物化学尚未得到评估。然而，这些糖蛋白显示出与免疫球蛋白G、寡克隆免疫球蛋白G、副蛋白或常规阳离子血清蛋白不同。与标准糖蛋白相比，大致推测其碳水化合物含量为30±10%。凝集素结合证明寡糖可能含有唾液酸。尽管诊断各异，但具有这种糖蛋白模式的患者中有90%患有炎症过程。
