神经系统疾病中脑脊液寡克隆IgM带的分布：琼脂糖电泳与等电聚焦的比较
Distribution of cerebrospinal fluid oligoclonal IgM bands in neurological diseases: a comparison between agarose electrophoresis and isoelectric focusing.
作者信息
Sharief M K, Thompson E J
机构信息
Department of Clinical Neurochemistry, Institute of Neurology, Queen Square, London, UK.
出版信息
J Neurol Sci. 1992 May;109(1):83-7. doi: 10.1016/0022-510x(92)90098-6.
Isoelectric focusing (IEF) and agarose gel electrophoresis were used to detect oligoclonal IgM bands in cerebrospinal fluid and serum samples from 850 patients with diverse neurological diseases. Oligoclonal IgM bands in cerebrospinal fluid were mainly detected in patients with infectious and inflammatory disorders of the nervous system. Both IEF and agarose electrophoresis revealed similar frequencies of oligoclonal IgM bands. Bands detected by IEF were mainly seen in the anodal range. Despite higher resolving capacity, IEF was less specific than agarose gel electrophoresis. It is concluded that oligoclonal IgM bands have important diagnostic significance and that agarose gel electrophoresis is more suitable for their detection in routine clinical work and use in differential diagnosis.
采用等电聚焦（IEF）和琼脂糖凝胶电泳检测850例患有各种神经系统疾病患者的脑脊液和血清样本中的寡克隆IgM条带。脑脊液中的寡克隆IgM条带主要在患有神经系统感染性和炎症性疾病的患者中检测到。IEF和琼脂糖电泳显示寡克隆IgM条带的出现频率相似。IEF检测到的条带主要出现在阳极区域。尽管IEF具有更高的分辨率，但特异性低于琼脂糖凝胶电泳。结论是寡克隆IgM条带具有重要的诊断意义，并且琼脂糖凝胶电泳更适合在常规临床工作中检测它们并用于鉴别诊断。
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