van Houten Bernd H G W, van Doesburg Wim, Dijkman Henk, Copini Cris, Smidt Hauke, Stams Alfons J M
Laboratory of Microbiology, Dreijenplein 10, 6703 HB, Wageningen, The Netherlands.
Appl Microbiol Biotechnol. 2009 Sep;84(3):555-63. doi: 10.1007/s00253-009-2075-8. Epub 2009 Jun 19.
The performance of a full-scale (500 m3) sulfidogenic synthesis gas fed gas-lift reactor treating metal- and sulfate-rich wastewater was investigated over a period of 128 weeks. After startup, the reactor had a high methanogenic activity of 46 Nm3.h(-1). Lowering the carbon dioxide feed rate during the first 6 weeks gradually lowered the methane production rate. Between weeks 8 and 93, less than 1% of the hydrogen supplied was used for methanogenesis. Denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified 16S rRNA gene fragments showed that the archaeal community decreased in diversity but did not disappear completely. After the carbon dioxide feed rate increased in week 88, the methane production rate also increased, confirming that methane production was carbon dioxide limited. Even though lowering the carbon dioxide feed appeared to affect part of the sulfate-reducing community, it did not prevent achieving the desired rates of sulfate reduction. The average sulfate conversion rate was 181 kg.h(-1) for the first 92 weeks. After 92 weeks, the sulfate input rate was increased and from week 94 to 128, the average weekly sulfate conversion rate was 295 kg.h(-1) (SD+/-87). Even higher sulfate conversion rates of up to 400 kg.h(-1) could be sustained for weeks 120-128. The long-term performance and stability together with the ability to control methanogenesis demonstrates that synthesis gas fed reactor can be used successfully at full scale to treat metal and sulfate-rich wastewater.
对一个全尺寸（500立方米）的合成气进料气升式反应器进行了为期128周的研究，该反应器用于处理富含金属和硫酸盐的废水。启动后，该反应器具有46 Nm³·h⁻¹的高产甲烷活性。在最初的6周内降低二氧化碳进料速率，逐渐降低了甲烷产率。在第8周和第93周之间，供应的氢气中不到1%用于产甲烷。对聚合酶链反应扩增的16S rRNA基因片段进行变性梯度凝胶电泳分析表明，古菌群落多样性降低，但并未完全消失。在第88周二氧化碳进料速率增加后，甲烷产率也增加，证实甲烷产生受二氧化碳限制。尽管降低二氧化碳进料似乎影响了部分硫酸盐还原菌群，但并未妨碍达到所需的硫酸盐还原速率。在前92周，平均硫酸盐转化率为181 kg·h⁻¹。92周后，增加了硫酸盐输入速率，从第94周到第128周，平均每周硫酸盐转化率为295 kg·h⁻¹（标准差±87）。在第120 - 128周期间，甚至可以维持高达400 kg·h⁻¹的更高硫酸盐转化率。长期性能和稳定性以及控制产甲烷的能力表明，合成气进料反应器可成功用于全尺寸处理富含金属和硫酸盐的废水。
