Zácek Petr, Kalinová Blanka, Sobotník Jan, Hovorka Oldrich, Ptácek Vladimír, Coppée Audrey, Verheggen François, Valterová Irena
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 166 10, Prague, Czech Republic.
J Chem Ecol. 2009 Jun;35(6):698-705. doi: 10.1007/s10886-009-9650-4. Epub 2009 Jun 20.
Age-related changes of antennal-active components of male labial gland extracts were studied in two closely related bumblebee species, Bombus terrestris and B. lucorum. In B. terrestris, compounds eliciting electroantennogram (EAG) responses of virgin queens were ethyl dodecanoate, 2,3-dihydrofarnesal, 2,3-dihydrofarnesol, hexadecan-1-ol, octadeca-9,12,15-trien-1-ol, and geranylcitronellol. Compounds that elicited EAG responses from queens of B. lucorum were ethyl dodecanoate, ethyl tetradec-7-enoate, ethyl tetradec-9-enoate, ethyl hexadec-9-enoate, hexadecan-1-ol, hexadec-7-enal, octadeca-9,12-dien-1-ol, octadeca-9,12,15-trien-1-ol, and octadecan-1-ol. Quantities of these compounds in the labial glands changed significantly over the lifetime of the respective males of the two species. In both species, concentrations of the respective compounds reached their maximum within seven days after eclosion. Subsequently, a rapid decrease in the amount of EAG-active compounds occurred in B. terrestris, whereas in B. lucorum the amount of active compounds stayed approximately constant or decreased at a slow rate. Microscopy showed that in B. terrestris secretory cells of the labial glands undergo apoptosis from the fifth to the tenth day of life, whilst in B. lucorum labial gland cells remain unchanged throughout the life of the males.
在两种亲缘关系密切的熊蜂物种——地熊蜂和亮熊蜂中，研究了雄性唇腺提取物触角活性成分的年龄相关变化。在地熊蜂中，能引起未交配蜂王触角电图（EAG）反应的化合物有十二烷酸乙酯、2,3 - 二氢法尼醛、2,3 - 二氢法尼醇、十六烷 - 1 - 醇、9,12,15 - 十八碳三烯 - 1 - 醇和香叶基香茅醇。能引起亮熊蜂蜂王EAG反应的化合物有十二烷酸乙酯、十四碳 - 7 - 烯酸乙酯、十四碳 - 9 - 烯酸乙酯、十六碳 - 9 - 烯酸乙酯、十六烷 - 1 - 醇、十六碳 - 7 - 烯醛、9,12 - 十八碳二烯 - 1 - 醇、9,12,15 - 十八碳三烯 - 1 - 醇和十八烷 - 1 - 醇。这两种熊蜂雄性个体一生中，这些化合物在唇腺中的含量发生了显著变化。在这两个物种中，各自化合物的浓度在羽化后七天内达到最大值。随后，地熊蜂中EAG活性化合物的量迅速减少，而在亮熊蜂中，活性化合物的量大致保持恒定或缓慢减少。显微镜检查表明，地熊蜂唇腺的分泌细胞在生命的第五天到第十天会发生凋亡，而在亮熊蜂中，唇腺细胞在雄性个体的整个生命过程中保持不变。
