Assunção Marco, Santos-Marques Maria J, Monteiro Rosário, Azevedo Isabel, Andrade José P, Carvalho Félix, Martins Maria J
Department of Anatomy (U121/94-FCT), Faculty of Medicine, University of Porto, Alameda Professor Hernâni Monteiro, 4200-319 Porto, Portugal.
J Agric Food Chem. 2009 Jul 22;57(14):6066-73. doi: 10.1021/jf900576h.
Ethanol consumption may be deleterious to the liver. However, alcoholic beverages contain, besides ethanol (EtOH), complex chemical mixtures that can modify EtOH's adverse effects. Red wine (RW) is rich in polyphenolic antioxidants, often reported as hepatoprotective agents. This study aimed to investigate the effects of 6 months of RW ingestion on hepatic oxidative stress and inflammation. Six-month-old Wistar rats were treated with RW or EtOH; controls were pair-fed. EtOH increased 8-hydroxy-2'-deoxyguanosine and decreased reduced and oxidized glutathione. These animals also displayed stimulated superoxide dismutase, catalase, and glutathione reductase activities. RW treatment decreased malondialdehyde and reduced glutathione levels. Glutathione-S-transferase and selenium-dependent glutathione peroxidase activities were stimulated and glutathione reductase activity was inhibited by RW intake. No modifications were detected in nuclear factor-kappa B or alkaline phosphatase activities. EtOH consumption induced fibrosis in portal spaces and hepatocyte lipid accumulation that were absent with RW treatment. This paper highlights the importance of RW nonalcoholic components and the relevance of biological matrix in the study of EtOH oxidative effects.
摄入乙醇可能对肝脏有害。然而，除了乙醇（EtOH）之外，酒精饮料还含有复杂的化学混合物，这些混合物可以改变乙醇的不良影响。红酒（RW）富含多酚类抗氧化剂，常被报道为具有肝脏保护作用的物质。本研究旨在探讨6个月饮用红酒对肝脏氧化应激和炎症的影响。对6月龄的Wistar大鼠进行红酒或乙醇处理；对照组采用配对喂养。乙醇使8-羟基-2'-脱氧鸟苷增加，并使还原型和氧化型谷胱甘肽减少。这些动物还表现出超氧化物歧化酶、过氧化氢酶和谷胱甘肽还原酶活性增强。红酒处理可降低丙二醛和还原型谷胱甘肽水平。红酒摄入可刺激谷胱甘肽-S-转移酶和硒依赖性谷胱甘肽过氧化物酶活性，并抑制谷胱甘肽还原酶活性。未检测到核因子-κB或碱性磷酸酶活性的改变。摄入乙醇会导致门管区纤维化和肝细胞脂质蓄积，而红酒处理则不会出现这种情况。本文强调了红酒非酒精成分的重要性以及生物基质在乙醇氧化作用研究中的相关性。
