Department of Genetic Medicine and Development, Geneva University Medical School, Geneva, Switzerland.
Reprod Toxicol. 2009 Dec;28(4):536-41. doi: 10.1016/j.reprotox.2009.06.009. Epub 2009 Jun 21.
Some steps of the assisted reproduction techniques, such as superovulation, may interfere with imprinting reprogramming. In the present study, superovulation was induced in the mouse and its possible effects on the differentially methylated domains of 2 paternally (H19 and Gtl2) and 3 maternally (Peg1, Snrpn and Peg3) imprinted genes were tested in the male offspring over 2 generations. The CpGs methylation status was analyzed by pyro- and bisulfite sequencing. In liver, skeletal muscle and tail, no effect of superovulation could be observed. In the sperm, however, a significant 6% decrease in the number of methylated CpGs of H19 and significant 2.8- and 7.0-fold increases in those of Peg1 and Snrpn, respectively were observed following superovulation. The changes were still present in the H19 and Snrpn genes of the second generation offspring. This suggests that superovulation in the mother transgenerationally affects the offspring sperm methylation pattern.
一些辅助生殖技术步骤，如超排卵，可能会干扰印迹重编程。在本研究中，在小鼠中诱导超排卵，并在雄性后代的 2 代中测试其对 2 个父系（H19 和 Gtl2）和 3 个母系（Peg1、Snrpn 和 Peg3）印记基因的差异甲基化域的可能影响。通过焦磷酸测序和亚硫酸氢盐测序分析 CpG 甲基化状态。在肝脏、骨骼肌和尾巴中，超排卵没有观察到影响。然而，在精子中，H19 的甲基化 CpG 数量显著减少了 6%，Peg1 和 Snrpn 的甲基化 CpG 数量分别显著增加了 2.8 倍和 7.0 倍。这些变化在第二代后代的 H19 和 Snrpn 基因中仍然存在。这表明母亲的超排卵会跨代影响后代精子的甲基化模式。
