Robaszkiewicz M, Reid B J, Volant A, Cauvin J M, Rabinovitch P S, Gouerou H
Department of Gastroenterology, University Hospital, Brest, France.
Gastroenterology. 1991 Dec;101(6):1588-93. doi: 10.1016/0016-5085(91)90396-3.
To better understand the mechanisms of esophageal carcinogenesis, abnormalities in DNA content of esophageal squamous cell carcinomas were studied. Cellular DNA content was determined by flow cytometric study of 70 endoscopic biopsy specimens obtained from 26 patients with esophageal squamous carcinoma. High-quality histograms were obtained for 23 patients. Twenty-one patients had at least one aneuploid population in their tumor. In 7 patients, multiple aneuploid peaks were detected. Specimens from 2 patients were diploid. The interpretation of the DNA histograms was difficult in 3 patients; an aneuploid population of cells was probable in 2 of them. A statistically significant relationship was found between the degree of differentiation and DNA content abnormalities in the regions of the tumors that could be evaluated by endoscopic biopsies: well-differentiated carcinomas had diploid or small aneuploid populations containing less than 15% of the cells, whereas DNA histograms of moderately or poorly differentiated carcinomas were characterized by large aneuploid peaks representing 25%-90% of the cells and a higher proliferative fraction. No relationship was found between the size or the stage of the tumor and the DNA content detected in endoscopic biopsy samples. The frequency and the multiplicity of abnormal clones in esophageal squamous carcinomas indicates that this cancer, like esophageal adenocarcinoma, develops an association with an acquired genomic instability that produces abnormal clones of cells, according to the multistep model of neoplastic progression.
为了更好地理解食管癌变的机制，对食管鳞状细胞癌的DNA含量异常进行了研究。通过流式细胞术对从26例食管鳞状细胞癌患者获取的70份内镜活检标本进行细胞DNA含量测定。23例患者获得了高质量的直方图。21例患者的肿瘤中至少有一个非整倍体群体。7例患者检测到多个非整倍体峰。2例患者的标本为二倍体。3例患者的DNA直方图难以解读；其中2例可能存在非整倍体细胞群体。在内镜活检可评估的肿瘤区域，分化程度与DNA含量异常之间存在统计学上的显著关系：高分化癌为二倍体或小非整倍体群体，其中细胞含量少于15%，而中分化或低分化癌的DNA直方图特征为大非整倍体峰，占细胞的25%-90%，且增殖分数较高。肿瘤大小或分期与内镜活检样本中检测到的DNA含量之间未发现相关性。食管鳞状细胞癌中异常克隆的频率和多样性表明，根据肿瘤进展的多步骤模型，这种癌症与食管腺癌一样，会发展出与获得性基因组不稳定性的关联，从而产生异常的细胞克隆。
