Ducruet Jean-Marc, Vass Imre
Groupe de Biospectroscopie Végétale, Ecologie Systématique Evolution, Université Paris-Sud-Orsay & Institut National Recherche Agronomique, Orsay, France.
Photosynth Res. 2009 Aug-Sep;101(2-3):195-204. doi: 10.1007/s11120-009-9436-0. Epub 2009 Jun 24.
Thermoluminesence measurements are useful for the study of Photosystem II electron transport in intact leaves, in algal and cyanobacterial cells, as well as in isolated membrane complexes. Here an overview of the experimental approaches is provided. In the present review, instruments and the experimental procedures for measuring thermoluminescence emission from photosynthetic systems of various origins are summarized and discussed. Major pitfalls frequently encountered in measurements with isolated membranes, suspensions of intact organisms or solid leaf samples are highlighted. Analytical and numeric methods for the analysis of measured thermoluminescence curves are also discussed.
热发光测量对于研究完整叶片、藻类和蓝细菌细胞以及分离的膜复合物中的光系统II电子传递很有用。本文提供了实验方法的概述。在本综述中,总结并讨论了用于测量各种来源光合系统热发光发射的仪器和实验程序。强调了在使用分离膜、完整生物体悬浮液或固体叶片样品进行测量时经常遇到的主要陷阱。还讨论了分析测量的热发光曲线的分析方法和数值方法。