基于真菌的银纳米颗粒合成及其掺入海藻酸钠薄膜用于蔬菜和水果保鲜。

Mycobased synthesis of silver nanoparticles and their incorporation into sodium alginate films for vegetable and fruit preservation.

作者信息

Mohammed Fayaz A, Balaji K, Girilal M, Kalaichelvan P T, Venkatesan R

机构信息

CAS in Botany, University of Madras, Guindy Campus, Chennai, India.

出版信息

J Agric Food Chem. 2009 Jul 22;57(14):6246-52. doi: 10.1021/jf900337h.

DOI:10.1021/jf900337h

PMID:19552418

Abstract

Biosynthesis of silver nanoparticles using Trichoderma viride and their incorporation into sodium alginate for vegetable and fruit preservation has been demonstrated in this study. Aqueous silver (Ag(+)) ions when exposed to the filtrate of T. viride are reduced in solution. These extremely stable silver nanoparticles were characterized by means of UV-vis spectrophotometer, FTIR, TEM, and EDS. The nanoparticles exhibit maximum absorbance at 421 nm in the UV spectrum. The presence of proteins was identified by FTIR. TEM micrograph revealed the formation of polydispersed nanoparticles, and the presence of elemental silver was confirmed by EDS analysis. The silver nanoparticle incorporated sodium alginate thin film shows good antibacterial activity against test strains. This film increases the shelf life of carrot and pear when compared to control with respect to weight loss and soluble protein content. These results show silver nanoparticle incorporated sodium alginate coated vegetables and fruits are suitable for preservation.

摘要

本研究证明了利用绿色木霉生物合成银纳米颗粒并将其掺入海藻酸钠用于蔬菜和水果保鲜。当暴露于绿色木霉的滤液时，水溶液中的银（Ag(+)）离子在溶液中被还原。这些极其稳定的银纳米颗粒通过紫外可见分光光度计、傅里叶变换红外光谱仪、透射电子显微镜和能谱仪进行了表征。纳米颗粒在紫外光谱中于421nm处表现出最大吸光度。通过傅里叶变换红外光谱仪鉴定了蛋白质的存在。透射电子显微镜照片显示形成了多分散的纳米颗粒，能谱分析证实了元素银的存在。掺入银纳米颗粒的海藻酸钠薄膜对测试菌株显示出良好的抗菌活性。与对照相比，该薄膜在重量损失和可溶性蛋白质含量方面延长了胡萝卜和梨的货架期。这些结果表明，掺入银纳米颗粒的海藻酸钠涂层蔬菜和水果适合保鲜。