CAS in Botany, University of Madras, Guindy Campus, Chennai 600 025, India.
Colloids Surf B Biointerfaces. 2010 Jan 1;75(1):175-8. doi: 10.1016/j.colsurfb.2009.08.028. Epub 2009 Aug 25.
Recent advances in nanomaterial have produced a new class of fluorescence labels by conjugating noble metal with biomolecules. The nanometer size metal conjugates are water soluble, biocompatible and provide important advantage over the fluorescence dyes. In this regard we synthesized silver nanoparticles at the size of 2-4 nm using biological route and studied fluorescence property of these nanoparticles. We observe that these silver (Ag(+)) ions when exposed to filtrate of Trichoderma viride are reduced in solution, thereby leading to the formation of an extremely stable silver hydrosol. These silver nanoparticles were characterized by means of UV-vis spectrophotometer, FTIR, HrTEM, EDX, XRD and fluorescence spectroscopy. The nanoparticles exhibit maximum absorbance at 405 nm in UV-vis spectrum. The presence of proteins was identified by FTIR. The HrTEM micrograph revealed the formation of monodispersed spherical nanoparticles and the presence of elemental silver was confirmed by EDX analysis and XRD. These monodispersed silver nanoparticles showed emission in the range of 320-520 nm wavelength.
近年来，纳米材料的发展通过将贵金属与生物分子结合，产生了一类新型的荧光标记物。纳米尺寸的金属结合物具有水溶性、生物相容性，并提供了比荧光染料更重要的优势。在这方面，我们使用生物途径合成了尺寸为 2-4nm 的银纳米粒子，并研究了这些纳米粒子的荧光性质。我们观察到，这些银（Ag(+））离子在暴露于绿色木霉滤液时在溶液中被还原，从而导致极其稳定的银水溶胶的形成。这些银纳米粒子通过紫外-可见分光光度计、傅里叶变换红外光谱仪、高分辨率透射电子显微镜、能谱仪、X 射线衍射仪和荧光光谱仪进行了表征。纳米粒子在紫外-可见光谱中在 405nm 处显示出最大吸收。傅里叶变换红外光谱仪鉴定了蛋白质的存在。高分辨率透射电子显微镜照片显示形成了单分散的球形纳米粒子，通过能谱仪分析和 X 射线衍射证实了元素银的存在。这些单分散的银纳米粒子在 320-520nm 波长范围内显示出发射。
