• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Dihydrofolate reductase gene amplification-associated shift of differentiation in methotrexate-adapted HT-29 cells.甲氨蝶呤适应的HT-29细胞中，二氢叶酸还原酶基因扩增相关的分化转变
J Cell Biol. 1991 Dec;115(5):1409-18. doi: 10.1083/jcb.115.5.1409.
2
Growth adaptation to methotrexate of HT-29 human colon carcinoma cells is associated with their ability to differentiate into columnar absorptive and mucus-secreting cells.HT-29人结肠癌细胞对甲氨蝶呤的生长适应性与其分化为柱状吸收细胞和黏液分泌细胞的能力有关。
Cancer Res. 1990 Oct 1;50(19):6334-43.
3
Adaptation to 5-fluorouracil of the heterogeneous human colon tumor cell line HT-29 results in the selection of cells committed to differentiation.人结肠肿瘤异质细胞系HT - 29对5 - 氟尿嘧啶的适应性导致了致力于分化的细胞的选择。
Int J Cancer. 1991 Nov 11;49(5):721-30. doi: 10.1002/ijc.2910490516.
4
Properties of an altered dihydrofolate reductase encoded by amplified genes in cultured mouse fibroblasts.培养的小鼠成纤维细胞中扩增基因编码的改变的二氢叶酸还原酶的特性
J Biol Chem. 1981 Sep 25;256(18):9501-10.
5
Amplified dihydrofolate reductase genes in unstably methotrexate-resistant cells are associated with double minute chromosomes.在不稳定的甲氨蝶呤抗性细胞中扩增的二氢叶酸还原酶基因与双微体染色体相关。
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5669-73. doi: 10.1073/pnas.76.11.5669.
6
Rapid spontaneous dihydrofolate reductase gene amplification shown by fluorescence-activated cell sorting.通过荧光激活细胞分选显示的快速自发二氢叶酸还原酶基因扩增
Proc Natl Acad Sci U S A. 1983 Jun;80(12):3711-5. doi: 10.1073/pnas.80.12.3711.
7
Resistance of the human colon carcinoma cell line HCT-8 to methotrexate results in selection of cells with features of enterocytic differentiation.人结肠癌细胞系HCT - 8对甲氨蝶呤的耐药性导致具有肠细胞分化特征的细胞被筛选出来。
Int J Cancer. 1998 Mar 2;75(5):731-7. doi: 10.1002/(sici)1097-0215(19980302)75:5<731::aid-ijc11>3.0.co;2-9.
8
Stimulation of methotrexate resistance and dihydrofolate reductase gene amplification by c-myc.c-myc对甲氨蝶呤耐药性的诱导及二氢叶酸还原酶基因扩增
Oncogene. 1991 Aug;6(8):1453-7.
9
5-fluorouracil modulation of dihydrofolate reductase RNA levels in methotrexate-resistant KB cells.5-氟尿嘧啶对甲氨蝶呤耐药KB细胞中二氢叶酸还原酶RNA水平的调节作用
J Biol Chem. 1983 Nov 10;258(21):13299-306.
10
The use of a fluorescent methotrexate probe to monitor the effects of three vinca alkaloids on a mixed population of parental L1210 and gene-amplified methotrexate-resistant cells by flow cytometry.使用荧光甲氨蝶呤探针，通过流式细胞术监测三种长春花生物碱对亲本L1210和基因扩增的甲氨蝶呤耐药细胞混合群体的影响。
Cancer Chemother Pharmacol. 1984;13(1):54-7. doi: 10.1007/BF00401448.

引用本文的文献

1
technology and ADMET research in traditional Chinese medicine.中药中的技术与ADMET研究
Front Pharmacol. 2025 Jul 9;16:1605330. doi: 10.3389/fphar.2025.1605330. eCollection 2025.
2
Caco-2 Cell Sheet Partially Laminated with HT29-MTX Cells as a Novel In Vitro Model of Gut Epithelium Drug Permeability.以部分层叠有HT29-MTX细胞的Caco-2细胞片作为肠道上皮药物渗透性的新型体外模型
Pharmaceutics. 2023 Sep 18;15(9):2338. doi: 10.3390/pharmaceutics15092338.
3
Metabolism of Caprine Milk Carbohydrates by Probiotic Bacteria and Caco-2:HT29⁻MTX Epithelial Co-Cultures and Their Impact on Intestinal Barrier Integrity.山羊乳碳水化合物的益生菌代谢及 Caco-2:HT29⁻MTX 肠上皮细胞共培养物及其对肠道屏障完整性的影响。
Nutrients. 2018 Jul 23;10(7):949. doi: 10.3390/nu10070949.
4
Effect of a Semi-Purified Oligosaccharide-Enriched Fraction from Caprine Milk on Barrier Integrity and Mucin Production of Co-Culture Models of the Small and Large Intestinal Epithelium.山羊奶半纯化富含寡糖组分对小肠和大肠上皮共培养模型屏障完整性和粘蛋白产生的影响。
Nutrients. 2016 May 6;8(5):267. doi: 10.3390/nu8050267.
5
Application of Caco-2 cell line in herb-drug interaction studies: current approaches and challenges.Caco-2 细胞系在中药-药物相互作用研究中的应用：当前方法和挑战。
J Pharm Pharm Sci. 2014;17(1):1-19. doi: 10.18433/j30k63.
6
Fate of paclitaxel lipid nanocapsules in intestinal mucus in view of their oral delivery.考虑到紫杉醇脂质纳米胶囊的口服递送，其在肠道黏液中的命运。
Int J Nanomedicine. 2013;8:4291-302. doi: 10.2147/IJN.S51837. Epub 2013 Nov 7.
7
Protein expression profiles of intestinal epithelial co-cultures: effect of functionalised carbon nanotube exposure.肠道上皮共培养物的蛋白质表达谱：功能化碳纳米管暴露的影响。
Int J Biomed Nanosci Nanotechnol. 2013;3(1-2). doi: 10.1504/IJBNN.2013.054508.
8
Proteomic profiling of halloysite clay nanotube exposure in intestinal cell co-culture.埃洛石粘土纳米管暴露于肠道细胞共培养体系中的蛋白质组学分析
J Appl Toxicol. 2013 Nov;33(11):1316-29. doi: 10.1002/jat.2858. Epub 2013 Apr 22.
9
Development of a serum-free co-culture of human intestinal epithelium cell-lines (Caco-2/HT29-5M21).人肠上皮细胞系（Caco-2/HT29-5M21）无血清共培养体系的建立。
BMC Cell Biol. 2006 May 2;7:20. doi: 10.1186/1471-2121-7-20.
10
Characterization of a spontaneously polarizing HT-29 cell line, HT-29/cl.f8.一种自发极化的HT-29细胞系HT-29/cl.f8的特性分析
In Vitro Cell Dev Biol Anim. 2004 Nov-Dec;40(10):297-302. doi: 10.1290/04100061.1.

本文引用的文献

1
STUDIES ON THE INHIBITION OF DIHYDROFOLATE REDUCTASE BY THE FOLATE ANTAGONISTS.叶酸拮抗剂对二氢叶酸还原酶抑制作用的研究。
J Biol Chem. 1964 Feb;239:479-85.
2
Intronless human dihydrofolate reductase genes are derived from processed RNA molecules.
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7435-9. doi: 10.1073/pnas.79.23.7435.
3
A mechanism of resistance to methotrexate. NADPH but not NADH stimulation of methotrexate binding to dihydrofolate reductase.一种对甲氨蝶呤的耐药机制。NADPH而非NADH刺激甲氨蝶呤与二氢叶酸还原酶的结合。
Biochem Pharmacol. 1983 Jun 15;32(12):1837-41. doi: 10.1016/0006-2952(83)90047-3.
4
Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate.丁酸钠处理后，人结肠癌细胞系在培养中出现永久分化的细胞克隆。
Cancer Res. 1984 Sep;44(9):3961-9.
5
Amplification and organization of dihydrofolate reductase genes in a human leukemic cell line, K-562, resistant to methotrexate.在对甲氨蝶呤耐药的人白血病细胞系K-562中，二氢叶酸还原酶基因的扩增与组织。
Biochemistry. 1983 Dec 6;22(25):5774-81. doi: 10.1021/bi00294a015.
6
Epithelial properties of human colonic carcinoma cell line Caco-2: electrical parameters.人结肠癌细胞系Caco-2的上皮特性：电参数
Am J Physiol. 1984 Sep;247(3 Pt 1):C260-7. doi: 10.1152/ajpcell.1984.247.3.C260.
7
Gene amplification in cultured animal cells.培养动物细胞中的基因扩增。
Cell. 1984 Jul;37(3):705-13. doi: 10.1016/0092-8674(84)90406-9.
8
The pharmacology and clinical use of methotrexate.甲氨蝶呤的药理学与临床应用。
N Engl J Med. 1983 Nov 3;309(18):1094-104. doi: 10.1056/NEJM198311033091805.
9
Chromosomal organization of the human dihydrofolate reductase genes: dispersion, selective amplification, and a novel form of polymorphism.
Proc Natl Acad Sci U S A. 1984 Aug;81(16):5170-4. doi: 10.1073/pnas.81.16.5170.
10
Origin, differentiation and renewal of the four main epithelial cell types in the mouse small intestine. V. Unitarian Theory of the origin of the four epithelial cell types.小鼠小肠中四种主要上皮细胞类型的起源、分化和更新。V. 四种上皮细胞类型起源的统一理论。
Am J Anat. 1974 Dec;141(4):537-61. doi: 10.1002/aja.1001410407.

甲氨蝶呤适应的HT-29细胞中，二氢叶酸还原酶基因扩增相关的分化转变

Dihydrofolate reductase gene amplification-associated shift of differentiation in methotrexate-adapted HT-29 cells.

作者信息

Lesuffleur T, Barbat A, Luccioni C, Beaumatin J, Clair M, Kornowski A, Dussaulx E, Dutrillaux B, Zweibaum A

机构信息

Unité de Recherches sur la Différenciation Cellulaire Intestinale, INSERM U178, Villejuif, France.

出版信息

J Cell Biol. 1991 Dec;115(5):1409-18. doi: 10.1083/jcb.115.5.1409.

DOI:10.1083/jcb.115.5.1409
PMID:1955481
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2289245/
Abstract

Postconfluent cultures of HT-29 cells form a heterogeneous multilayer of which greater than 95% of the cells are undifferentiated. In contrast, when stably adapted to normally lethal concentrations of methotrexate (10(-6)-10(-5) M), they form a monolayer of gobletlike cells (Lesuffleur et al., 1990) which secrete large quantities of mucins and display a discrete brush border with the presence of villin, dipeptidylpeptidase-IV, and carcinoembryonic antigen. When adapted to even higher concentrations of methotrexate (10(-4) and 10(-3) M) there is a shift in the pattern of differentiation from gobletlike to dome-forming absorptive-like cells. These cells still display an apical brush border which expresses villin and dipeptidylpeptidase-IV, but no longer express significant levels of mucins and carcinoembryonic antigen. This shift of differentiation coincides with a sudden amplification of the gene coding for dihydrofolate reductase and an increased activity of the enzyme.

摘要

HT - 29细胞汇合后培养形成异质多层结构，其中超过95%的细胞未分化。相比之下，当稳定适应正常致死浓度的甲氨蝶呤（10^(-6)-10^(-5) M）时，它们形成单层杯状细胞（勒叙弗勒等人，1990年），这些细胞分泌大量粘蛋白，并呈现出含有绒毛蛋白、二肽基肽酶-IV和癌胚抗原的离散刷状缘。当适应更高浓度的甲氨蝶呤（10^(-4)和10^(-3) M）时，分化模式从杯状细胞转变为形成穹顶的吸收样细胞。这些细胞仍然显示出表达绒毛蛋白和二肽基肽酶-IV的顶端刷状缘，但不再表达大量的粘蛋白和癌胚抗原。这种分化转变与编码二氢叶酸还原酶的基因突然扩增以及该酶活性增加相吻合。