Elovaara I, Seppälä I, Kinnunen E, Laaksovirta H
Department of Microbiology, Aurora Hospital, Helsinki, Finland.
J Neuroimmunol. 1991 Dec;35(1-3):65-77. doi: 10.1016/0165-5728(91)90162-z.
The presence of free immunoglobulin light chains (FLCs) in the cerebrospinal fluid (CSF) and sera of patients with human immunodeficiency virus-1 (HIV-1) infection, multiple sclerosis (MS), and neurologically healthy control individuals was investigated by paying special attention to ensure that only truly free light chains would be detected. The FLCs were extracted by specifically binding them to Sepharose-coupled anti-FLC monoclonal antibodies, and thereafter they were electrophoresed and immunoblotted with monoclonal antibodies to both light chain (LC) isotypes. A frequent occurrence of kappa and lambda FLCs was found in both CSF and sera of HIV-1 infected patients. In HIV-1 infection and in MS, the frequency of FLCs of the CSF was equal. In healthy controls, only occasional weak FLCs were observed in either CSF or serum. FLC bands of the CSF from patients with HIV-1 infection tended to be more intensive than those of the appropriately diluted sera. Both intrathecal synthesis of FLCs and their transudation from sera through the impaired blood-brain barrier (BBB) may contribute to this. Increasing severity of general HIV-1 infection was accompanied by an increase of FLC intensity in sera. A qualitative demonstration of FLC in the CSF may be meaningful only in the absence of altered BBB function.
研究了人类免疫缺陷病毒1型（HIV-1）感染患者、多发性硬化症（MS）患者以及神经功能正常的对照个体的脑脊液（CSF）和血清中游离免疫球蛋白轻链（FLC）的存在情况，特别注意确保仅检测到真正的游离轻链。通过将FLC特异性结合到琼脂糖偶联的抗FLC单克隆抗体上来提取FLC，然后对其进行电泳并用针对两种轻链（LC）同种型的单克隆抗体进行免疫印迹。在HIV-1感染患者的脑脊液和血清中均频繁发现κ和λFLC。在HIV-1感染和MS中，脑脊液中FLC的频率相等。在健康对照中，在脑脊液或血清中仅偶尔观察到弱阳性的FLC。HIV-1感染患者脑脊液中的FLC条带往往比适当稀释的血清中的条带更强。这可能是由于FLC的鞘内合成及其通过受损血脑屏障（BBB）从血清中的渗出共同导致的。全身性HIV-1感染严重程度的增加伴随着血清中FLC强度的增加。仅在血脑屏障功能未改变的情况下，脑脊液中FLC的定性检测才可能有意义。
