Gordon David E, Mirza Myriam, Sahlender Daniela A, Jakovleska Jovana, Peden Andrew A
Department of Clinical Biochemistry, University of Cambridge, Cambridge, UK.
EMBO Rep. 2009 Aug;10(8):851-6. doi: 10.1038/embor.2009.96. Epub 2009 Jun 26.
The sorting of post-Golgi R-SNAREs (vesicle-associated membrane protein (VAMP)1, 2, 3, 4, 7 and 8) is still poorly understood. To address this, we developed a system to investigate their localization, trafficking and cell-surface levels. Here, we show that the distribution and internalization of VAMPs 3 and 8 are determined solely through a new conserved mechanism that uses coiled-coil interactions, and that VAMP4 does not require these interactions for its trafficking. We propose that VAMPs 3 and 8 are trafficked while in a complex with Q-SNAREs. We also show that the dileucine motif of VAMP4 is required for both its internalization and retrieval to the trans-Golgi network. However, when the dileucine motif is mutated, the construct can still be internalized potentially through coiled-coil interactions with Q-SNAREs.
高尔基体后R-SNARE蛋白（囊泡相关膜蛋白（VAMP）1、2、3、4、7和8）的分选机制仍未得到充分了解。为了解决这个问题，我们开发了一个系统来研究它们的定位、运输和细胞表面水平。在这里，我们表明VAMP 3和8的分布和内化完全由一种新的保守机制决定，该机制利用卷曲螺旋相互作用，而VAMP 4的运输不需要这些相互作用。我们提出VAMP 3和8在与Q-SNARE蛋白形成复合物时被运输。我们还表明，VAMP 4的双亮氨酸基序对于其内化和返回反式高尔基体网络都是必需的。然而，当双亮氨酸基序发生突变时，构建体仍可能通过与Q-SNARE蛋白的卷曲螺旋相互作用而被内化。
