Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, USA.
Am J Infect Control. 2009 Nov;37(9):729-33. doi: 10.1016/j.ajic.2009.03.010. Epub 2009 Jun 25.
Health care-associated infections because of methicillin-resistant strains of Staphylococcus aureus (MRSA) are increasing worldwide despite current infection control measures. Novel methods for disinfection of MRSA would be useful.
We tested the effectiveness of atmospheric, non-thermal plasma discharge at killing S aureus, including USA300 strains, and at disinfecting experimentally contaminated hospital pagers.
Exposure of S aureus to plasma at different concentrations and for varying lengths of time resulted in up to a 4- to 5-log(10) kill on tryptic soy agar plates within 10 minutes and was not toxic to epithelial cells. USA300 strains of MRSA were more resistant to plasma-based killing than other tested strains. Disinfection of hospital pagers experimentally coated with clinically relevant amounts of MRSA could be achieved in as little as 30 seconds.
Generation of plasma is a promising method for disinfection of objects or surfaces that warrants further study in hospital settings. The USA300 strains of S aureus may be more resistant to disinfection than other strains.
尽管目前采取了感染控制措施，但由于耐甲氧西林金黄色葡萄球菌（MRSA）的卫生保健相关感染在全球范围内仍呈上升趋势。新型的 MRSA 消毒方法将是有用的。
我们测试了大气压非热等离子体放电杀死包括 USA300 株在内的金黄色葡萄球菌以及对实验污染的医院寻呼机进行消毒的效果。
金黄色葡萄球菌在不同浓度和不同时间的等离子体暴露下，在 10 分钟内可使胰蛋白酶大豆琼脂平板上的细菌减少 4-5 对数，且对上皮细胞无毒。与其他测试株相比，MRSA 的 USA300 株对基于等离子体的杀伤更具抵抗力。仅需 30 秒即可实现对实验涂层有临床相关数量的 MRSA 的医院寻呼机的消毒。
等离子体的产生是一种有前途的物体或表面消毒方法，值得在医院环境中进一步研究。与其他菌株相比，USA300 株金黄色葡萄球菌可能对消毒更具抵抗力。
