Departamento de Ciências Básicas da Saúde, Universidade Estadual de Maringá, PR, Brazil; Hospital Universitário de Maringá, Universidade Estadual de Maringá, PR, Brazil.
Universidade Federal de São Paulo, São Paulo, SP, Brazil.
J Hosp Infect. 2015 Aug;90(4):347-50. doi: 10.1016/j.jhin.2015.04.005. Epub 2015 Apr 30.
Contaminated surfaces play an important role in the transmission of certain pathogens that are responsible for healthcare-associated infections. Although previous studies have shown that meticillin-resistant Staphylococcus aureus (MRSA) can survive on dry surfaces at room temperature, no published data regarding vancomycin-intermediate S. aureus (VISA) are available to date.
To compare the survival time on different types of surfaces, cell-surface hydrophobicity, adherence to abiotic surfaces and biofilm formation of meticillin-susceptible S. aureus (MSSA), MRSA and VISA.
Survival of the S. aureus strains was tested on latex, cotton fabric, vinyl flooring and formica. Cell-surface hydrophobicity was determined using the hydrocarbon interaction affinity method. Adhesion to abiotic surfaces was tested on granite, latex (gloves), glass, vinyl flooring and formica. Biofilm formation was evaluated at 6, 12, 24 and 48 h.
All of the samples survived on the vinyl flooring and formica for at least 40 days. VISA survived on both surfaces for more than 45 days. All of the strains were highly hydrophobic. VISA adhered to latex, vinyl flooring and formica. Biofilm formation increased for all of the tested strains within 6-24 h.
VISA present high survival, adherence and cell-surface hydrophobicity. Therefore, as the treatment of patients with VISA is a significant challenge for clinicians, greater care with cleaning and disinfection of different types of surfaces in healthcare facilities is recommended because these may become important reservoirs of multi-resistant pathogens.
污染表面在某些病原体的传播中起着重要作用，这些病原体是导致医院感染的原因。虽然之前的研究表明，耐甲氧西林金黄色葡萄球菌（MRSA）可以在室温下的干燥表面存活，但目前还没有关于中间葡萄球菌（VISA）的发表数据。
比较不同类型表面上的存活时间、细胞表面疏水性、对非生物表面的黏附以及耐甲氧西林金黄色葡萄球菌（MSSA）、MRSA 和 VISA 的生物膜形成。
在乳胶、棉织物、乙烯基地板和防火板上测试金黄色葡萄球菌菌株的存活情况。使用烃相互作用亲和力法测定细胞表面疏水性。在花岗岩、乳胶（手套）、玻璃、乙烯基地板和防火板上测试对非生物表面的黏附。在 6、12、24 和 48 小时评估生物膜形成。
所有样本在乙烯基地板和防火板上至少存活 40 天。VISA 在这两种表面上的存活时间超过 45 天。所有菌株均具有高度疏水性。VISA 黏附于乳胶、乙烯基地板和防火板。所有测试菌株的生物膜形成在 6-24 小时内增加。
VISA 具有高存活、黏附和细胞表面疏水性。因此，由于治疗 VISA 患者对临床医生来说是一个重大挑战，因此建议在医疗机构中对不同类型的表面进行更严格的清洁和消毒，因为这些表面可能成为多耐药病原体的重要储存库。
